A synthetic DNA molecule in three knotted topologies

Shou Ming Du, Nadrian Seeman, Nadrian C. Seeman

Research output: Contribution to journalArticle

Abstract

The construction of knotted topologies is a key goal of stereochemistry. In order to measure the chiral properties of knotted molecules, it is necessary to produce both enantiomers of a knot from the same molecule. A molecule containing the same backbone structure that is an amphichiral knot can provide a useful control molecule for such measurements. In the case of molecules with chiral backbones, configurational chirality, exclusive of the chirality due to knotting, must be measured from the circle of the same sequence. Trefoil knots of both chiralities, an amphichiral knot, and an unknotted circular molecule have all been constructed by enzymatic closure of the same linear DNA molecule. The molecule contains two double helical domains that can be induced to assume the right-handed B conformation or the left-handed Z conformation under selected solution conditions. The molecules expected to contain left-handed DNA have been shown to bind an anti-Z-DNA antibody in gel-retention assays.

Original languageEnglish (US)
Pages (from-to)1194-1200
Number of pages7
JournalJournal of the American Chemical Society
Volume117
Issue number4
DOIs
StatePublished - Feb 1 1995

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Z-Form DNA
DNA
Topology
Molecules
Antinuclear Antibodies
Chirality
Gels
Conformations
Stereochemistry
Enantiomers
Antibodies
Assays

Keywords

  • control of polymer structure
  • DNA branched junctions
  • DNA knots
  • DNA topology

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

A synthetic DNA molecule in three knotted topologies. / Du, Shou Ming; Seeman, Nadrian; Seeman, Nadrian C.

In: Journal of the American Chemical Society, Vol. 117, No. 4, 01.02.1995, p. 1194-1200.

Research output: Contribution to journalArticle

Du, Shou Ming ; Seeman, Nadrian ; Seeman, Nadrian C. / A synthetic DNA molecule in three knotted topologies. In: Journal of the American Chemical Society. 1995 ; Vol. 117, No. 4. pp. 1194-1200.
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