A 1H NMR Assay for measuring the photostationary states of photoswitchable ligands

Matthew R. Banghart, Dirk Trauner

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Incorporation of photoisomerizable chromophores into small molecule ligands represents a general approach for reversibly controlling protein function with light. Illumination at different wavelengths produces photostationary states (PSSs) consisting of different ratios of photoisomers. Thus optimal implementation of photoswitchable ligands requires knowledge of their wavelength sensitivity. Using an azobenzene-based ion channel blocker as an example, this protocol describes a 1H NMR assay that can be used to precisely determine the isomeric content of photostationary states (PSSs) as a function of illumination wavelength. Samples of the photoswitchable ligand are dissolved in deuterated water and analyzed by UV/VIS spectroscopy to identify the range of illumination wavelengths that produce PSSs. The PSSs produced by these wavelengths are quantified using 1H NMR spectroscopy under continuous irradiation through a monochromator-coupled fiber-optic cable. Because aromatic protons of azobenzene trans and cis isomers exhibit sufficiently different chemical shifts, their relative abundances at each PSS can be readily determined by peak integration. Constant illumination during spectrum acquisition is essential to accurately determine PSSs from molecules that thermally relax on the timescale of minutes or faster. This general protocol can be readily applied to any photoswitch that exhibits distinct 1H NMR signals in each photoisomeric state.

Original languageEnglish (US)
Title of host publicationChemical Neurobiology
Subtitle of host publicationMethods and Protocols
Pages107-120
Number of pages14
Volume995
DOIs
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
Volume995
ISSN (Print)1064-3745

Fingerprint

Lighting
Ligands
Optical Fibers
Ion Channels
Protons
Spectrum Analysis
Magnetic Resonance Spectroscopy
Light
Proton Magnetic Resonance Spectroscopy
Water
Proteins
azobenzene

Keywords

  • Azobenzene
  • NMR
  • Photoisomerization
  • Photostationary state
  • Photoswitch

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Banghart, M. R., & Trauner, D. (2013). A 1H NMR Assay for measuring the photostationary states of photoswitchable ligands. In Chemical Neurobiology: Methods and Protocols (Vol. 995, pp. 107-120). (Methods in Molecular Biology; Vol. 995). https://doi.org/10.1007/978-1-62703-345-9_8

A 1H NMR Assay for measuring the photostationary states of photoswitchable ligands. / Banghart, Matthew R.; Trauner, Dirk.

Chemical Neurobiology: Methods and Protocols. Vol. 995 2013. p. 107-120 (Methods in Molecular Biology; Vol. 995).

Research output: Chapter in Book/Report/Conference proceedingChapter

Banghart, MR & Trauner, D 2013, A 1H NMR Assay for measuring the photostationary states of photoswitchable ligands. in Chemical Neurobiology: Methods and Protocols. vol. 995, Methods in Molecular Biology, vol. 995, pp. 107-120. https://doi.org/10.1007/978-1-62703-345-9_8
Banghart MR, Trauner D. A 1H NMR Assay for measuring the photostationary states of photoswitchable ligands. In Chemical Neurobiology: Methods and Protocols. Vol. 995. 2013. p. 107-120. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-62703-345-9_8
Banghart, Matthew R. ; Trauner, Dirk. / A 1H NMR Assay for measuring the photostationary states of photoswitchable ligands. Chemical Neurobiology: Methods and Protocols. Vol. 995 2013. pp. 107-120 (Methods in Molecular Biology).
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