A set of BAC clones spanning the human genome

Martin Krzywinski, Ian Bosdet, Duane Smailus, Readman Chiu, Carrie Mathewson, Natasja Wye, Sarah Barber, Mabel Brown-John, Susanna Chan, Steve Chand, Alison Cloutier, Noreen Girn, Darlene Lee, Amara Masson, Michael Mayo, Teika Olson, Pawan Pandoh, Anna Liisa Prabhu, Eric Schoenmakers, Miranda TsaiDonna Albertson, Wan Lam, Chik On Choy, Kazutoyo Osoegawa, Shaying Zhao, Pieter J. de Jong, Jacqueline Schein, Steven Jones, Marco A. Marra

Research output: Contribution to journalArticle

Abstract

Using the human bacterial artificial chromosome (BAC) fingerprint-based physical map, genome sequence assembly and BAC end sequences, we have generated a fingerprint-validated set of 32 855 BAC clones spanning the human genome. The clone set provides coverage for at least 98% of the human fingerprint map, 99% of the current assembled sequence and has an effective resolving power of 79 kb. We have made the clone set publicly available, anticipating that it will generally facilitate FISH or array-CGH-based identification and characterization of chromosomal alterations relevant to disease.

Original languageEnglish (US)
Pages (from-to)3651-3660
Number of pages10
JournalNucleic Acids Research
Volume32
Issue number12
DOIs
StatePublished - 2004

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Bacterial Artificial Chromosomes
Dermatoglyphics
Human Genome
Clone Cells
Human Artificial Chromosomes
Genome

ASJC Scopus subject areas

  • Genetics

Cite this

Krzywinski, M., Bosdet, I., Smailus, D., Chiu, R., Mathewson, C., Wye, N., ... Marra, M. A. (2004). A set of BAC clones spanning the human genome. Nucleic Acids Research, 32(12), 3651-3660. https://doi.org/10.1093/nar/gkh700

A set of BAC clones spanning the human genome. / Krzywinski, Martin; Bosdet, Ian; Smailus, Duane; Chiu, Readman; Mathewson, Carrie; Wye, Natasja; Barber, Sarah; Brown-John, Mabel; Chan, Susanna; Chand, Steve; Cloutier, Alison; Girn, Noreen; Lee, Darlene; Masson, Amara; Mayo, Michael; Olson, Teika; Pandoh, Pawan; Prabhu, Anna Liisa; Schoenmakers, Eric; Tsai, Miranda; Albertson, Donna; Lam, Wan; Choy, Chik On; Osoegawa, Kazutoyo; Zhao, Shaying; de Jong, Pieter J.; Schein, Jacqueline; Jones, Steven; Marra, Marco A.

In: Nucleic Acids Research, Vol. 32, No. 12, 2004, p. 3651-3660.

Research output: Contribution to journalArticle

Krzywinski, M, Bosdet, I, Smailus, D, Chiu, R, Mathewson, C, Wye, N, Barber, S, Brown-John, M, Chan, S, Chand, S, Cloutier, A, Girn, N, Lee, D, Masson, A, Mayo, M, Olson, T, Pandoh, P, Prabhu, AL, Schoenmakers, E, Tsai, M, Albertson, D, Lam, W, Choy, CO, Osoegawa, K, Zhao, S, de Jong, PJ, Schein, J, Jones, S & Marra, MA 2004, 'A set of BAC clones spanning the human genome', Nucleic Acids Research, vol. 32, no. 12, pp. 3651-3660. https://doi.org/10.1093/nar/gkh700
Krzywinski M, Bosdet I, Smailus D, Chiu R, Mathewson C, Wye N et al. A set of BAC clones spanning the human genome. Nucleic Acids Research. 2004;32(12):3651-3660. https://doi.org/10.1093/nar/gkh700
Krzywinski, Martin ; Bosdet, Ian ; Smailus, Duane ; Chiu, Readman ; Mathewson, Carrie ; Wye, Natasja ; Barber, Sarah ; Brown-John, Mabel ; Chan, Susanna ; Chand, Steve ; Cloutier, Alison ; Girn, Noreen ; Lee, Darlene ; Masson, Amara ; Mayo, Michael ; Olson, Teika ; Pandoh, Pawan ; Prabhu, Anna Liisa ; Schoenmakers, Eric ; Tsai, Miranda ; Albertson, Donna ; Lam, Wan ; Choy, Chik On ; Osoegawa, Kazutoyo ; Zhao, Shaying ; de Jong, Pieter J. ; Schein, Jacqueline ; Jones, Steven ; Marra, Marco A. / A set of BAC clones spanning the human genome. In: Nucleic Acids Research. 2004 ; Vol. 32, No. 12. pp. 3651-3660.
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AU - Barber, Sarah

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AU - Jones, Steven

AU - Marra, Marco A.

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