A set of BAC clones spanning the human genome

Martin Krzywinski, Ian Bosdet, Duane Smailus, Readman Chiu, Carrie Mathewson, Natasja Wye, Sarah Barber, Mabel Brown-John, Susanna Chan, Steve Chand, Alison Cloutier, Noreen Girn, Darlene Lee, Amara Masson, Michael Mayo, Teika Olson, Pawan Pandoh, Anna Liisa Prabhu, Eric Schoenmakers, Miranda Tsai & 9 others Donna Albertson, Wan Lam, Chik On Choy, Kazutoyo Osoegawa, Shaying Zhao, Pieter J. de Jong, Jacqueline Schein, Steven Jones, Marco A. Marra

Research output: Contribution to journalArticle

Abstract

Using the human bacterial artificial chromosome (BAC) fingerprint-based physical map, genome sequence assembly and BAC end sequences, we have generated a fingerprint-validated set of 32 855 BAC clones spanning the human genome. The clone set provides coverage for at least 98% of the human fingerprint map, 99% of the current assembled sequence and has an effective resolving power of 79 kb. We have made the clone set publicly available, anticipating that it will generally facilitate FISH or array-CGH-based identification and characterization of chromosomal alterations relevant to disease.

Original languageEnglish (US)
Pages (from-to)3651-3660
Number of pages10
JournalNucleic Acids Research
Volume32
Issue number12
DOIs
StatePublished - 2004

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Bacterial Artificial Chromosomes
Dermatoglyphics
Human Genome
Clone Cells
Human Artificial Chromosomes
Genome

ASJC Scopus subject areas

  • Genetics

Cite this

Krzywinski, M., Bosdet, I., Smailus, D., Chiu, R., Mathewson, C., Wye, N., ... Marra, M. A. (2004). A set of BAC clones spanning the human genome. Nucleic Acids Research, 32(12), 3651-3660. https://doi.org/10.1093/nar/gkh700

A set of BAC clones spanning the human genome. / Krzywinski, Martin; Bosdet, Ian; Smailus, Duane; Chiu, Readman; Mathewson, Carrie; Wye, Natasja; Barber, Sarah; Brown-John, Mabel; Chan, Susanna; Chand, Steve; Cloutier, Alison; Girn, Noreen; Lee, Darlene; Masson, Amara; Mayo, Michael; Olson, Teika; Pandoh, Pawan; Prabhu, Anna Liisa; Schoenmakers, Eric; Tsai, Miranda; Albertson, Donna; Lam, Wan; Choy, Chik On; Osoegawa, Kazutoyo; Zhao, Shaying; de Jong, Pieter J.; Schein, Jacqueline; Jones, Steven; Marra, Marco A.

In: Nucleic Acids Research, Vol. 32, No. 12, 2004, p. 3651-3660.

Research output: Contribution to journalArticle

Krzywinski, M, Bosdet, I, Smailus, D, Chiu, R, Mathewson, C, Wye, N, Barber, S, Brown-John, M, Chan, S, Chand, S, Cloutier, A, Girn, N, Lee, D, Masson, A, Mayo, M, Olson, T, Pandoh, P, Prabhu, AL, Schoenmakers, E, Tsai, M, Albertson, D, Lam, W, Choy, CO, Osoegawa, K, Zhao, S, de Jong, PJ, Schein, J, Jones, S & Marra, MA 2004, 'A set of BAC clones spanning the human genome', Nucleic Acids Research, vol. 32, no. 12, pp. 3651-3660. https://doi.org/10.1093/nar/gkh700
Krzywinski M, Bosdet I, Smailus D, Chiu R, Mathewson C, Wye N et al. A set of BAC clones spanning the human genome. Nucleic Acids Research. 2004;32(12):3651-3660. https://doi.org/10.1093/nar/gkh700
Krzywinski, Martin ; Bosdet, Ian ; Smailus, Duane ; Chiu, Readman ; Mathewson, Carrie ; Wye, Natasja ; Barber, Sarah ; Brown-John, Mabel ; Chan, Susanna ; Chand, Steve ; Cloutier, Alison ; Girn, Noreen ; Lee, Darlene ; Masson, Amara ; Mayo, Michael ; Olson, Teika ; Pandoh, Pawan ; Prabhu, Anna Liisa ; Schoenmakers, Eric ; Tsai, Miranda ; Albertson, Donna ; Lam, Wan ; Choy, Chik On ; Osoegawa, Kazutoyo ; Zhao, Shaying ; de Jong, Pieter J. ; Schein, Jacqueline ; Jones, Steven ; Marra, Marco A. / A set of BAC clones spanning the human genome. In: Nucleic Acids Research. 2004 ; Vol. 32, No. 12. pp. 3651-3660.
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AU - Barber, Sarah

AU - Brown-John, Mabel

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AU - Jones, Steven

AU - Marra, Marco A.

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