A role for frizzled 3 in neural crest development

M. A. Deardorff, C. Tan, Jean-Pierre Saint-Jeannet, P. S. Klein

Research output: Contribution to journalArticle

Abstract

Wnts are a large family of secreted molecules implicated in numerous developmental processes. Frizzled proteins are likely receptors for Wnts and are required for Wnt signaling in invertebrates. A large number of vertebrate frizzled genes have also been identified, but their roles in mediating specific responses to endogenous Wnts have not been well defined. Using a functional assay in Xenopus, we have performed a large screen to identify potential interactions between Wnts and frizzleds. We find that signaling by Xwnt1, but not other Wnts, can be specifically enhanced by frizzled 3 (Xfz3). As both Xfz3 and Xwnt1 are highly localized to dorsal neural tissues that give rise to neural crest, we examined whether Xfz3 mediates Xwnt1 signaling in the formation of neural crest. Xfz3 specifically induces neural crest in ectodermal explants and in embryos, similar to Xwnt1, and at lower levels of expression, synergizes with Xwnt1 in neural crest induction. Furthermore, loss of Xfz3 function, either by depletion with a Xfz3-directed morpholino antisense oligonucleotide or by expression of an inhibitory form of Xfz3 (Nfz3), prevents Xwnt1-dependent neural crest induction in ectodermal explants and blocks neural crest formation in whole embryos. These results show that Xfz3 is required for Xwnt1 signaling in the formation of the neural crest in the developing vertebrate embryo.

Original languageEnglish (US)
Pages (from-to)3655-3663
Number of pages9
JournalDevelopment
Volume128
Issue number19
StatePublished - 2001

Fingerprint

Neural Crest
Embryonic Structures
Vertebrates
Frizzled Receptors
Morpholinos
Antisense Oligonucleotides
Invertebrates
Xenopus
Genes

Keywords

  • Frizzled
  • Melanocyte
  • Neural crest
  • Slug
  • Vertebrate embryo
  • Xenopus
  • Xfz3
  • Xwnt

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Deardorff, M. A., Tan, C., Saint-Jeannet, J-P., & Klein, P. S. (2001). A role for frizzled 3 in neural crest development. Development, 128(19), 3655-3663.

A role for frizzled 3 in neural crest development. / Deardorff, M. A.; Tan, C.; Saint-Jeannet, Jean-Pierre; Klein, P. S.

In: Development, Vol. 128, No. 19, 2001, p. 3655-3663.

Research output: Contribution to journalArticle

Deardorff, MA, Tan, C, Saint-Jeannet, J-P & Klein, PS 2001, 'A role for frizzled 3 in neural crest development', Development, vol. 128, no. 19, pp. 3655-3663.
Deardorff MA, Tan C, Saint-Jeannet J-P, Klein PS. A role for frizzled 3 in neural crest development. Development. 2001;128(19):3655-3663.
Deardorff, M. A. ; Tan, C. ; Saint-Jeannet, Jean-Pierre ; Klein, P. S. / A role for frizzled 3 in neural crest development. In: Development. 2001 ; Vol. 128, No. 19. pp. 3655-3663.
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