A RHAMM mimetic peptide blocks hyaluronan signaling and reduces inflammation and fibrogenesis in excisional skin wounds

Cornelia Tolg, Sara R. Hamilton, Ewa Zalinska, Lori McCulloch, Ripal Amin, Natalia Akentieva, Francoise Winnik, Rashmin Savani, Darius J. Bagli, Len G. Luyt, Mary Cowman, Jim B. McCarthy, Eva A. Turley

Research output: Contribution to journalArticle

Abstract

Hyaluronan is activated by fragmentation and controls inflammation and fibroplasia during wound repair and diseases (eg, cancer). Hyaluronan-binding peptides were identified that modify fibrogenesis during skin wound repair. Peptides were selected from 7- to 15mer phage display libraries by panning with hyaluronan-Sepharose beads and assayed for their ability to block fibroblast migration in response to hyaluronan oligosaccharides (10 kDa). A 15mer peptide (P15-1), with homology to receptor for hyaluronan mediated motility (RHAMM) hyaluronan binding sequences, was the most effective inhibitor. P15-1 bound to 10-kDa hyaluronan with an affinity of Kd = 10-7 and appeared to specifically mimic RHAMM since it significantly reduced binding of hyaluronan oligosaccharides to recombinant RHAMM but not to recombinant CD44 or TLR2,4, and altered wound repair in wild-type but not RHAMM-/- mice. One topical application of P15-1 to full-thickness excisional rat wounds significantly reduced wound macrophage number, fibroblast number, and blood vessel density compared to scrambled, negative control peptides. Wound collagen 1, transforming growth factor β-1, and α-smooth muscle actin were reduced, whereas tenascin C was increased, suggesting that P15-1 promoted a form of scarless healing. Signaling/microarray analyses showed that P15-1 blocks RHAMM-regulated focal adhesion kinase pathways in fibroblasts. These results identify a new class of reagents that attenuate proinflammatory, fibrotic repair by blocking hyaluronan oligosaccharide signaling.

Original languageEnglish (US)
Pages (from-to)1250-1270
Number of pages21
JournalAmerican Journal of Pathology
Volume181
Issue number4
DOIs
StatePublished - Oct 2012

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Hyaluronic Acid
Inflammation
Skin
Peptides
Wounds and Injuries
Oligosaccharides
Fibroblasts
Tenascin
Focal Adhesion Protein-Tyrosine Kinases
hyaluronan-mediated motility receptor
Transforming Growth Factors
Microarray Analysis
Bacteriophages
Sepharose
Libraries
Smooth Muscle
Blood Vessels
Actins
Collagen
Macrophages

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Tolg, C., Hamilton, S. R., Zalinska, E., McCulloch, L., Amin, R., Akentieva, N., ... Turley, E. A. (2012). A RHAMM mimetic peptide blocks hyaluronan signaling and reduces inflammation and fibrogenesis in excisional skin wounds. American Journal of Pathology, 181(4), 1250-1270. https://doi.org/10.1016/j.ajpath.2012.06.036

A RHAMM mimetic peptide blocks hyaluronan signaling and reduces inflammation and fibrogenesis in excisional skin wounds. / Tolg, Cornelia; Hamilton, Sara R.; Zalinska, Ewa; McCulloch, Lori; Amin, Ripal; Akentieva, Natalia; Winnik, Francoise; Savani, Rashmin; Bagli, Darius J.; Luyt, Len G.; Cowman, Mary; McCarthy, Jim B.; Turley, Eva A.

In: American Journal of Pathology, Vol. 181, No. 4, 10.2012, p. 1250-1270.

Research output: Contribution to journalArticle

Tolg, C, Hamilton, SR, Zalinska, E, McCulloch, L, Amin, R, Akentieva, N, Winnik, F, Savani, R, Bagli, DJ, Luyt, LG, Cowman, M, McCarthy, JB & Turley, EA 2012, 'A RHAMM mimetic peptide blocks hyaluronan signaling and reduces inflammation and fibrogenesis in excisional skin wounds', American Journal of Pathology, vol. 181, no. 4, pp. 1250-1270. https://doi.org/10.1016/j.ajpath.2012.06.036
Tolg, Cornelia ; Hamilton, Sara R. ; Zalinska, Ewa ; McCulloch, Lori ; Amin, Ripal ; Akentieva, Natalia ; Winnik, Francoise ; Savani, Rashmin ; Bagli, Darius J. ; Luyt, Len G. ; Cowman, Mary ; McCarthy, Jim B. ; Turley, Eva A. / A RHAMM mimetic peptide blocks hyaluronan signaling and reduces inflammation and fibrogenesis in excisional skin wounds. In: American Journal of Pathology. 2012 ; Vol. 181, No. 4. pp. 1250-1270.
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