A ratiometric lectin microarray approach to analysis of the dynamic mammalian glycome

Kanoelani T. Pilobello, Deepika E. Slawek, Lara Mahal

Research output: Contribution to journalArticle

Abstract

Glycosylation creates an intricate and complex code for biological information that plays a role in cell-cell communication, infection, and immunity among many biological events. Dynamic changes in the glycosylation status of cells have been observed in tumor cell metastasis and cell differentiation but have been difficult to analyze because of a lack of high-throughput and facile technologies. Here, we present a method for the rapid evaluation of differences in the glycosylation of heterogeneous mammalian samples using a ratiometric two-color lectin microarray approach. This work represents a significant improvement in glycomics technology and sets the stage for the systematic evaluation of how glycans encode biological information in complex systems.

Original languageEnglish (US)
Pages (from-to)11534-11539
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume104
Issue number28
DOIs
StatePublished - Jul 10 2007

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Glycosylation
Lectins
Glycomics
Technology
Cell Communication
Polysaccharides
Cell Differentiation
Immunity
Color
Neoplasm Metastasis
Infection
Neoplasms

Keywords

  • Carbohydrate analysis
  • Glycomics

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

A ratiometric lectin microarray approach to analysis of the dynamic mammalian glycome. / Pilobello, Kanoelani T.; Slawek, Deepika E.; Mahal, Lara.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, No. 28, 10.07.2007, p. 11534-11539.

Research output: Contribution to journalArticle

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