A rapid method for the identification of ceramide activated protein phosphatase

Sehamuddin Galadari, K. Kishikawa

Research output: Contribution to journalArticle

Abstract

Recently, the sphingomyelin cycle has emerged as a key mechanism implicated in apoptosis, cell growth, differentiation, and cellular stress. This pathway is activated following Hgand binding of receptors and subsequent activation of a neutral cytosolic sphingomyelinase, which in turn, hydrolyzes sphingomyelin to generate ceramide and phosphorylcholin. The novel second messenger, ce ramide, once generated, acts through a ceramide activated protein phosphatase (CAPP) to elicit its downstream effects. Although, CAPP is a prime candidate for mediating ceramide signaling in mammalian cells, its identity and its connection to the downstream targets of ceramide activation remain elusive. Molecular characterization of CAPP is, therefore, a prelude to a better understanding of the mechanism by which ceramide causes its downstream effects. In this study we report a novel method for the separation and identification of CAPP. We have used a combination of ionexchange chromatography, hydrophobic inter action, and immunological methods, for the purification and identification of ('APP. Our studies demonstrate that CAPP consists of multimeric forms of protein phosphatase 2A (PP2A).

Original languageEnglish (US)
JournalFASEB Journal
Volume12
Issue number8
StatePublished - Dec 1 1998

Fingerprint

Ceramides
Phosphoprotein Phosphatases
Sphingomyelins
Hydrophobic chromatography
Chemical activation
Sphingomyelin Phosphodiesterase
Protein Phosphatase 2
Cell growth
Second Messenger Systems
Purification
Chromatography
Cell Differentiation
Cells
Apoptosis
Growth

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

A rapid method for the identification of ceramide activated protein phosphatase. / Galadari, Sehamuddin; Kishikawa, K.

In: FASEB Journal, Vol. 12, No. 8, 01.12.1998.

Research output: Contribution to journalArticle

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