A public genome-scale lentiviral expression library of human ORFs

Xiaoping Yang, Jesse S. Boehm, Xinping Yang, Kourosh Salehi-Ashtiani, Tong Hao, Yun Shen, Rakela Lubonja, Sapana R. Thomas, Ozan Alkan, Tashfeen Bhimdi, Thomas M. Green, Cory M. Johannessen, Serena J. Silver, Cindy Nguyen, Ryan R. Murray, Haley Hieronymus, Dawit Balcha, Changyu Fan, Chenwei Lin, Lila GhamsariMarc Vidal, William C. Hahn, David E. Hill, David E. Root

Research output: Contribution to journalArticle


Functional characterization of the human genome requires tools for systematically modulating gene expression in both loss-of-function and gain-of-function experiments. We describe the production of a sequence-confirmed, clonal collection of over 16,100 human open-reading frames (ORFs) encoded in a versatile Gateway vector system. Using this ORFeome resource, we created a genome-scale expression collection in a lentiviral vector, thereby enabling both targeted experiments and high-throughput screens in diverse cell types.

Original languageEnglish (US)
Pages (from-to)659-661
Number of pages3
JournalNature methods
Issue number8
StatePublished - Aug 1 2011


ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Yang, X., Boehm, J. S., Yang, X., Salehi-Ashtiani, K., Hao, T., Shen, Y., Lubonja, R., Thomas, S. R., Alkan, O., Bhimdi, T., Green, T. M., Johannessen, C. M., Silver, S. J., Nguyen, C., Murray, R. R., Hieronymus, H., Balcha, D., Fan, C., Lin, C., ... Root, D. E. (2011). A public genome-scale lentiviral expression library of human ORFs. Nature methods, 8(8), 659-661. https://doi.org/10.1038/nmeth.1638