A public genome-scale lentiviral expression library of human ORFs

Xiaoping Yang, Jesse S. Boehm, Xinping Yang, Kourosh Salehi-Ashtiani, Tong Hao, Yun Shen, Rakela Lubonja, Sapana R. Thomas, Ozan Alkan, Tashfeen Bhimdi, Thomas M. Green, Cory M. Johannessen, Serena J. Silver, Cindy Nguyen, Ryan R. Murray, Haley Hieronymus, Dawit Balcha, Changyu Fan, Chenwei Lin, Lila Ghamsari & 4 others Marc Vidal, William C. Hahn, David E. Hill, David E. Root

    Research output: Contribution to journalArticle

    Abstract

    Functional characterization of the human genome requires tools for systematically modulating gene expression in both loss-of-function and gain-of-function experiments. We describe the production of a sequence-confirmed, clonal collection of over 16,100 human open-reading frames (ORFs) encoded in a versatile Gateway vector system. Using this ORFeome resource, we created a genome-scale expression collection in a lentiviral vector, thereby enabling both targeted experiments and high-throughput screens in diverse cell types.

    Original languageEnglish (US)
    Pages (from-to)659-661
    Number of pages3
    JournalNature Methods
    Volume8
    Issue number8
    DOIs
    StatePublished - Aug 1 2011

    Fingerprint

    Human Genome
    Open Reading Frames
    Genes
    Genome
    Gene Expression
    Gene expression
    Experiments
    Throughput

    ASJC Scopus subject areas

    • Biotechnology
    • Biochemistry
    • Molecular Biology
    • Cell Biology

    Cite this

    Yang, X., Boehm, J. S., Yang, X., Salehi-Ashtiani, K., Hao, T., Shen, Y., ... Root, D. E. (2011). A public genome-scale lentiviral expression library of human ORFs. Nature Methods, 8(8), 659-661. https://doi.org/10.1038/nmeth.1638

    A public genome-scale lentiviral expression library of human ORFs. / Yang, Xiaoping; Boehm, Jesse S.; Yang, Xinping; Salehi-Ashtiani, Kourosh; Hao, Tong; Shen, Yun; Lubonja, Rakela; Thomas, Sapana R.; Alkan, Ozan; Bhimdi, Tashfeen; Green, Thomas M.; Johannessen, Cory M.; Silver, Serena J.; Nguyen, Cindy; Murray, Ryan R.; Hieronymus, Haley; Balcha, Dawit; Fan, Changyu; Lin, Chenwei; Ghamsari, Lila; Vidal, Marc; Hahn, William C.; Hill, David E.; Root, David E.

    In: Nature Methods, Vol. 8, No. 8, 01.08.2011, p. 659-661.

    Research output: Contribution to journalArticle

    Yang, X, Boehm, JS, Yang, X, Salehi-Ashtiani, K, Hao, T, Shen, Y, Lubonja, R, Thomas, SR, Alkan, O, Bhimdi, T, Green, TM, Johannessen, CM, Silver, SJ, Nguyen, C, Murray, RR, Hieronymus, H, Balcha, D, Fan, C, Lin, C, Ghamsari, L, Vidal, M, Hahn, WC, Hill, DE & Root, DE 2011, 'A public genome-scale lentiviral expression library of human ORFs', Nature Methods, vol. 8, no. 8, pp. 659-661. https://doi.org/10.1038/nmeth.1638
    Yang X, Boehm JS, Yang X, Salehi-Ashtiani K, Hao T, Shen Y et al. A public genome-scale lentiviral expression library of human ORFs. Nature Methods. 2011 Aug 1;8(8):659-661. https://doi.org/10.1038/nmeth.1638
    Yang, Xiaoping ; Boehm, Jesse S. ; Yang, Xinping ; Salehi-Ashtiani, Kourosh ; Hao, Tong ; Shen, Yun ; Lubonja, Rakela ; Thomas, Sapana R. ; Alkan, Ozan ; Bhimdi, Tashfeen ; Green, Thomas M. ; Johannessen, Cory M. ; Silver, Serena J. ; Nguyen, Cindy ; Murray, Ryan R. ; Hieronymus, Haley ; Balcha, Dawit ; Fan, Changyu ; Lin, Chenwei ; Ghamsari, Lila ; Vidal, Marc ; Hahn, William C. ; Hill, David E. ; Root, David E. / A public genome-scale lentiviral expression library of human ORFs. In: Nature Methods. 2011 ; Vol. 8, No. 8. pp. 659-661.
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