A protein-driven DNA device that measures the excess binding energy of proteins that distort DNA

Wanqiu Shen, Michael F. Bruist, Steven D. Goodman, Nadrian C. Seeman

Research output: Contribution to journalArticle

Abstract

A DNA nanomechanical device was constructed by linking two triple-helical DNA motifs with a shaft of conventional DNA bearing the binding site for the integration host factor (IHF, shown as green ellipse in the picture). Binding of IHF to this site causes marked shaft bending, which can be monitored by fluorescence resonance energy transfer between donor (D) and acceptor (A) dyes.

Original languageEnglish (US)
Pages (from-to)4750-4752
Number of pages3
JournalAngewandte Chemie - International Edition
Volume43
Issue number36
DOIs
Publication statusPublished - Sep 4 2004

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Keywords

  • DNA structures
  • Fluorescent probes
  • Molecular devices
  • Proteins

ASJC Scopus subject areas

  • Chemistry(all)

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