A PIIB-type Ca2+-ATPase is essential for stress adaptation in Physcomitrella patens

Enas Qudeimat, Alexander M C Faltusz, Glen Wheeler, Daniel Lang, Colin Brownlee, Ralf Reski, Wolfgang Frank

    Research output: Contribution to journalArticle

    Abstract

    Transient cytosolic Ca2+ ([Ca2+]cyt) elevations are early events in plant signaling pathways including those related to abiotic stress. The restoration of [Ca2+]cyt to prestimulus levels involves ATP-driven Ca2+ pumps, but direct evidence for an essential role of a plant Ca2+-ATPase in abiotic stress adaptation is missing. Here, we report on a stress-responsive Ca 2+-ATPase gene (PCA1) from the moss Physcomitrella patens. Functional analysis of PCA1 in a Ca2+ transport-deficient yeast mutant suggests that PCA1 encodes a PIIB-type Ca2+-ATPase harboring an N-terminal autoinhibitory domain. In vivo localizations identified membranes of small vacuoles as the integration site for a PCA1:GFP fusion protein. PCA1 mRNA levels are up-regulated by dehydration, NaCl, and abscisic acid, and PCA1 loss-of-function mutants (ΔPCA1) exhibit an enhanced susceptibility to salt stress. The ΔPCA1 lines show sustained elevated [Ca 2+]cyt in response to salt treatment in contrast to WT that shows transient Ca2+ elevations, indicating a direct role for PCA1 in the restoration of prestimulus [Ca2+]cyt. The altered Ca2+ response of the ΔPCA1 mutant lines correlates with altered expression levels of stress-induced genes, suggesting disturbance of a stress-associated signaling pathway. We propose that PCA1 is an essential component for abiotic stress adaptation in Physcomitrella involved in the generation of a specific salt-induced Ca2+ signature.

    Original languageEnglish (US)
    Pages (from-to)19555-19560
    Number of pages6
    JournalProceedings of the National Academy of Sciences of the United States of America
    Volume105
    Issue number49
    DOIs
    StatePublished - Dec 9 2008

    Fingerprint

    Bryopsida
    Calcium-Transporting ATPases
    Salts
    Bryophyta
    Abscisic Acid
    Vacuoles
    Dehydration
    Genes
    Adenosine Triphosphatases
    Adenosine Triphosphate
    Yeasts
    Messenger RNA
    Membranes
    Proteins

    Keywords

    • Abiotic stress
    • Calcium
    • Signaling
    • Targeted knockout

    ASJC Scopus subject areas

    • General

    Cite this

    A PIIB-type Ca2+-ATPase is essential for stress adaptation in Physcomitrella patens. / Qudeimat, Enas; Faltusz, Alexander M C; Wheeler, Glen; Lang, Daniel; Brownlee, Colin; Reski, Ralf; Frank, Wolfgang.

    In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, No. 49, 09.12.2008, p. 19555-19560.

    Research output: Contribution to journalArticle

    Qudeimat, Enas ; Faltusz, Alexander M C ; Wheeler, Glen ; Lang, Daniel ; Brownlee, Colin ; Reski, Ralf ; Frank, Wolfgang. / A PIIB-type Ca2+-ATPase is essential for stress adaptation in Physcomitrella patens. In: Proceedings of the National Academy of Sciences of the United States of America. 2008 ; Vol. 105, No. 49. pp. 19555-19560.
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