A novel peptide-producing cell in Xenopus

Multinucleated gastric mucosal cell strikingly similar to the granular gland of the skin

K. S. Moore, C. L. Bevins, N. Tomassini, K. M. Huttner, Kirsten Sadler Edepli, J. E. Moreira, J. Reynolds, M. Zasloff

    Research output: Contribution to journalArticle

    Abstract

    We have characterized a novel peptide-containing cell within the gastric mucosa of Xenopus laevis. The cell is a spherical, multinucleated syncytial structure containing a cytoplasmic space filled with dense rice-shaped granules, and is strikingly similar in morphology to the well-studied granular gland of the amphibian skin. Immunohistochemical and immunogold methods were used to demonstrate that several peptides previously isolated from the granular glands of the skin, including the antimicrobial peptides magainin and PGLa (a peptide with amino-terminal glycine and carboxy-terminal leucinamide), are also stored in granules present in these enteric cells. These data demonstrate that this enteric peptide-producing cell is strikingly similar both morphologically and biochemically to the granular gland, previously considered a highly specialized structure of the amphibian integument. This novel gastric mucosal cell, which we have designated a 'granular multinucleated cell,' is distinct in its morphology and its diversity of stored peptide products from other well-characterized peptide- containing cells in the vertebrate gastrointestinal tract.

    Original languageEnglish (US)
    Pages (from-to)367-378
    Number of pages12
    JournalJournal of Histochemistry and Cytochemistry
    Volume40
    Issue number3
    DOIs
    StatePublished - Jan 1 1992

    Fingerprint

    Xenopus
    Stomach
    Skin
    Peptides
    Amphibians
    Magainins
    Xenopus laevis
    Gastric Mucosa
    Glycine
    Vertebrates
    Gastrointestinal Tract

    Keywords

    • Antimicrobial peptides
    • Electron microscopy
    • Gastric mucosa
    • Granular multinucleated cell
    • Immunocytochemistry
    • Magainin
    • Xenopus

    ASJC Scopus subject areas

    • Anatomy
    • Histology

    Cite this

    A novel peptide-producing cell in Xenopus : Multinucleated gastric mucosal cell strikingly similar to the granular gland of the skin. / Moore, K. S.; Bevins, C. L.; Tomassini, N.; Huttner, K. M.; Sadler Edepli, Kirsten; Moreira, J. E.; Reynolds, J.; Zasloff, M.

    In: Journal of Histochemistry and Cytochemistry, Vol. 40, No. 3, 01.01.1992, p. 367-378.

    Research output: Contribution to journalArticle

    Moore, KS, Bevins, CL, Tomassini, N, Huttner, KM, Sadler Edepli, K, Moreira, JE, Reynolds, J & Zasloff, M 1992, 'A novel peptide-producing cell in Xenopus: Multinucleated gastric mucosal cell strikingly similar to the granular gland of the skin', Journal of Histochemistry and Cytochemistry, vol. 40, no. 3, pp. 367-378. https://doi.org/10.1177/40.3.1552176
    Moore, K. S. ; Bevins, C. L. ; Tomassini, N. ; Huttner, K. M. ; Sadler Edepli, Kirsten ; Moreira, J. E. ; Reynolds, J. ; Zasloff, M. / A novel peptide-producing cell in Xenopus : Multinucleated gastric mucosal cell strikingly similar to the granular gland of the skin. In: Journal of Histochemistry and Cytochemistry. 1992 ; Vol. 40, No. 3. pp. 367-378.
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