A novel peptide-producing cell in Xenopus: Multinucleated gastric mucosal cell strikingly similar to the granular gland of the skin

K. S. Moore, C. L. Bevins, N. Tomassini, K. M. Huttner, Kirsten Sadler Edepli, J. E. Moreira, J. Reynolds, M. Zasloff

Research output: Contribution to journalArticle

Abstract

We have characterized a novel peptide-containing cell within the gastric mucosa of Xenopus laevis. The cell is a spherical, multinucleated syncytial structure containing a cytoplasmic space filled with dense rice-shaped granules, and is strikingly similar in morphology to the well-studied granular gland of the amphibian skin. Immunohistochemical and immunogold methods were used to demonstrate that several peptides previously isolated from the granular glands of the skin, including the antimicrobial peptides magainin and PGLa (a peptide with amino-terminal glycine and carboxy-terminal leucinamide), are also stored in granules present in these enteric cells. These data demonstrate that this enteric peptide-producing cell is strikingly similar both morphologically and biochemically to the granular gland, previously considered a highly specialized structure of the amphibian integument. This novel gastric mucosal cell, which we have designated a 'granular multinucleated cell,' is distinct in its morphology and its diversity of stored peptide products from other well-characterized peptide- containing cells in the vertebrate gastrointestinal tract.

Original languageEnglish (US)
Pages (from-to)367-378
Number of pages12
JournalJournal of Histochemistry and Cytochemistry
Volume40
Issue number3
DOIs
StatePublished - Jan 1 1992

Fingerprint

Xenopus
Stomach
Skin
Peptides
Amphibians
Magainins
Xenopus laevis
Gastric Mucosa
Glycine
Vertebrates
Gastrointestinal Tract

Keywords

  • Antimicrobial peptides
  • Electron microscopy
  • Gastric mucosa
  • Granular multinucleated cell
  • Immunocytochemistry
  • Magainin
  • Xenopus

ASJC Scopus subject areas

  • Anatomy
  • Histology

Cite this

A novel peptide-producing cell in Xenopus : Multinucleated gastric mucosal cell strikingly similar to the granular gland of the skin. / Moore, K. S.; Bevins, C. L.; Tomassini, N.; Huttner, K. M.; Sadler Edepli, Kirsten; Moreira, J. E.; Reynolds, J.; Zasloff, M.

In: Journal of Histochemistry and Cytochemistry, Vol. 40, No. 3, 01.01.1992, p. 367-378.

Research output: Contribution to journalArticle

Moore, K. S. ; Bevins, C. L. ; Tomassini, N. ; Huttner, K. M. ; Sadler Edepli, Kirsten ; Moreira, J. E. ; Reynolds, J. ; Zasloff, M. / A novel peptide-producing cell in Xenopus : Multinucleated gastric mucosal cell strikingly similar to the granular gland of the skin. In: Journal of Histochemistry and Cytochemistry. 1992 ; Vol. 40, No. 3. pp. 367-378.
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