A novel, high conductance channel of mitochondria linked to apoptosis in mammalian cells and Bax expression in yeast

Evgeny Pavlov, Muriel Priault, Dawn Pietkiewicz, Emily H Y Cheng, Bruno Antonsson, Stephen Manon, Stanley J. Korsmeyer, Carmen A. Mannella, Kathleen W. Kinnally

Research output: Contribution to journalArticle

Abstract

During apoptosis, proapoptotic factors are released from mitochondria by as yet undefined mechanisms. Patch-clamping of mitochondria and proteoliposomes formed from mitochondrial outer membranes of mammalian (FL5.12) cells has uncovered a novel ion channel whose activity correlates with onset of apoptosis. The pore diameter inferred from the largest conductance state of this channel is ∼4 nm, sufficient to allow diffusion of cytochrome c and even larger proteins. The activity of the channel is affected by Bcl-2 family proteins in a manner consistent with their pro- or antiapoptotic properties. Thus, the channel activity correlates with presence of proapoptotic Bax in the mitochondrial outer membrane and is absent in mitochondria from cells overexpressing antiapoptotic Bcl-2. Also, a similar channel activity is found in mitochondrial outer membranes of yeast expressing human Bax. These findings implicate this channel, named mitochondrial apoptosis-induced channel, as a candidate for the outer-membrane pore through which cytochrome c and possibly other factors exit mitochondria during apoptosis.

Original languageEnglish (US)
Pages (from-to)725-731
Number of pages7
JournalJournal of Cell Biology
Volume155
Issue number5
DOIs
StatePublished - Nov 26 2001

Fingerprint

Mitochondria
Mitochondrial Membranes
Yeasts
Apoptosis
Cytochromes c
Ion Channels
Constriction
Proteins
Membranes

Keywords

  • Apoptosis
  • Bax
  • Channels
  • Mitochondria
  • Patch-clamp

ASJC Scopus subject areas

  • Cell Biology

Cite this

Pavlov, E., Priault, M., Pietkiewicz, D., Cheng, E. H. Y., Antonsson, B., Manon, S., ... Kinnally, K. W. (2001). A novel, high conductance channel of mitochondria linked to apoptosis in mammalian cells and Bax expression in yeast. Journal of Cell Biology, 155(5), 725-731. https://doi.org/10.1083/jcb.200107057

A novel, high conductance channel of mitochondria linked to apoptosis in mammalian cells and Bax expression in yeast. / Pavlov, Evgeny; Priault, Muriel; Pietkiewicz, Dawn; Cheng, Emily H Y; Antonsson, Bruno; Manon, Stephen; Korsmeyer, Stanley J.; Mannella, Carmen A.; Kinnally, Kathleen W.

In: Journal of Cell Biology, Vol. 155, No. 5, 26.11.2001, p. 725-731.

Research output: Contribution to journalArticle

Pavlov, E, Priault, M, Pietkiewicz, D, Cheng, EHY, Antonsson, B, Manon, S, Korsmeyer, SJ, Mannella, CA & Kinnally, KW 2001, 'A novel, high conductance channel of mitochondria linked to apoptosis in mammalian cells and Bax expression in yeast', Journal of Cell Biology, vol. 155, no. 5, pp. 725-731. https://doi.org/10.1083/jcb.200107057
Pavlov, Evgeny ; Priault, Muriel ; Pietkiewicz, Dawn ; Cheng, Emily H Y ; Antonsson, Bruno ; Manon, Stephen ; Korsmeyer, Stanley J. ; Mannella, Carmen A. ; Kinnally, Kathleen W. / A novel, high conductance channel of mitochondria linked to apoptosis in mammalian cells and Bax expression in yeast. In: Journal of Cell Biology. 2001 ; Vol. 155, No. 5. pp. 725-731.
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