A nanomechanical device based on the B-Z transition of DNA

Chengde Mao, Welqiong Sun, Zhiyong Shen, Nadrian Seeman

Research output: Contribution to journalArticle

Abstract

The assembly of synthetic, controllable molecular mechanical systems 1- 7 is one of the goals of nanotechnology. Protein-based molecular machines, often driven by an energy source such as ATP, are abundant in biology 8,9. It has been shown previously that branched motifs of DNA can provide components for the assembly of nanoscale objects 10, links 11 and arrays 12. Here we show that such structures can also provide the basis for dynamic assemblies: switchable molecular machines. We have constructed a supramolecular device consisting of two rigid DNA 'double-crossover' (DX) molecules connected by 4.5 double-helical turns. One domain of each DX molecule is attached to the connecting helix. To effect switchable motion in this assembly, we use the transition between the B and Z 13,14 forms of DNA. In conditions that favour B-DNA, the two unconnected domains of the DX molecules lie on the same side of the central helix. In Z-DNA-promoting conditions, however these domains switch to opposite sides of the helix. This relative repositioning is detected by means of fluorescence resonance energy transfer spectroscopy, which measures the relative proximity of two dye molecules attached to the free ends of the DX molecules. The switching event induces atomic displacement of 20-60 A9.

Original languageEnglish (US)
Pages (from-to)144-146
Number of pages3
JournalNature
Volume397
Issue number6715
DOIs
StatePublished - Jan 14 1999

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Z-Form DNA
Equipment and Supplies
DNA
Fluorescence Resonance Energy Transfer
Nucleotide Motifs
Nanotechnology
Spectrum Analysis
Coloring Agents
Adenosine Triphosphate
Proteins

ASJC Scopus subject areas

  • General

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A nanomechanical device based on the B-Z transition of DNA. / Mao, Chengde; Sun, Welqiong; Shen, Zhiyong; Seeman, Nadrian.

In: Nature, Vol. 397, No. 6715, 14.01.1999, p. 144-146.

Research output: Contribution to journalArticle

Mao, Chengde ; Sun, Welqiong ; Shen, Zhiyong ; Seeman, Nadrian. / A nanomechanical device based on the B-Z transition of DNA. In: Nature. 1999 ; Vol. 397, No. 6715. pp. 144-146.
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