A method for isolating and patch-clamping single mammalian taste receptor cells

Andrew Spielman, Istvan Mody, Joseph G. Brand, Glayde Whitney, John F. MacDonald, Michael W. Salter

Research output: Contribution to journalArticle

Abstract

Individual taste receptor cells were isolated from the tonque of the mouse by enzymatic treatment followed by mechanical dissociation. The cells were morphologically identical with taste cells from amphibians. Whole-cell voltage-clamp recordings indicated that the murine taste cells possess a variety of voltage-dependent inward and outward currents. Delayed rectifier currents were blocked by denatonium benzoate, one of the most bitter compounds known. This preparation should permit a detailed electrophysiological investigation of taste transduction in mammals at the level of taste receptor cells.

Original languageEnglish (US)
Pages (from-to)326-329
Number of pages4
JournalBrain Research
Volume503
Issue number2
DOIs
StatePublished - Dec 4 1989

Fingerprint

Constriction
Amphibians
Mammals

Keywords

  • Bitter
  • Denatonium benzoate
  • Taste cell
  • Whole-cell voltage-clamp

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Spielman, A., Mody, I., Brand, J. G., Whitney, G., MacDonald, J. F., & Salter, M. W. (1989). A method for isolating and patch-clamping single mammalian taste receptor cells. Brain Research, 503(2), 326-329. https://doi.org/10.1016/0006-8993(89)91684-3

A method for isolating and patch-clamping single mammalian taste receptor cells. / Spielman, Andrew; Mody, Istvan; Brand, Joseph G.; Whitney, Glayde; MacDonald, John F.; Salter, Michael W.

In: Brain Research, Vol. 503, No. 2, 04.12.1989, p. 326-329.

Research output: Contribution to journalArticle

Spielman, A, Mody, I, Brand, JG, Whitney, G, MacDonald, JF & Salter, MW 1989, 'A method for isolating and patch-clamping single mammalian taste receptor cells', Brain Research, vol. 503, no. 2, pp. 326-329. https://doi.org/10.1016/0006-8993(89)91684-3
Spielman, Andrew ; Mody, Istvan ; Brand, Joseph G. ; Whitney, Glayde ; MacDonald, John F. ; Salter, Michael W. / A method for isolating and patch-clamping single mammalian taste receptor cells. In: Brain Research. 1989 ; Vol. 503, No. 2. pp. 326-329.
@article{653f5a3464534e399139db6ac5d52de4,
title = "A method for isolating and patch-clamping single mammalian taste receptor cells",
abstract = "Individual taste receptor cells were isolated from the tonque of the mouse by enzymatic treatment followed by mechanical dissociation. The cells were morphologically identical with taste cells from amphibians. Whole-cell voltage-clamp recordings indicated that the murine taste cells possess a variety of voltage-dependent inward and outward currents. Delayed rectifier currents were blocked by denatonium benzoate, one of the most bitter compounds known. This preparation should permit a detailed electrophysiological investigation of taste transduction in mammals at the level of taste receptor cells.",
keywords = "Bitter, Denatonium benzoate, Taste cell, Whole-cell voltage-clamp",
author = "Andrew Spielman and Istvan Mody and Brand, {Joseph G.} and Glayde Whitney and MacDonald, {John F.} and Salter, {Michael W.}",
year = "1989",
month = "12",
day = "4",
doi = "10.1016/0006-8993(89)91684-3",
language = "English (US)",
volume = "503",
pages = "326--329",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - A method for isolating and patch-clamping single mammalian taste receptor cells

AU - Spielman, Andrew

AU - Mody, Istvan

AU - Brand, Joseph G.

AU - Whitney, Glayde

AU - MacDonald, John F.

AU - Salter, Michael W.

PY - 1989/12/4

Y1 - 1989/12/4

N2 - Individual taste receptor cells were isolated from the tonque of the mouse by enzymatic treatment followed by mechanical dissociation. The cells were morphologically identical with taste cells from amphibians. Whole-cell voltage-clamp recordings indicated that the murine taste cells possess a variety of voltage-dependent inward and outward currents. Delayed rectifier currents were blocked by denatonium benzoate, one of the most bitter compounds known. This preparation should permit a detailed electrophysiological investigation of taste transduction in mammals at the level of taste receptor cells.

AB - Individual taste receptor cells were isolated from the tonque of the mouse by enzymatic treatment followed by mechanical dissociation. The cells were morphologically identical with taste cells from amphibians. Whole-cell voltage-clamp recordings indicated that the murine taste cells possess a variety of voltage-dependent inward and outward currents. Delayed rectifier currents were blocked by denatonium benzoate, one of the most bitter compounds known. This preparation should permit a detailed electrophysiological investigation of taste transduction in mammals at the level of taste receptor cells.

KW - Bitter

KW - Denatonium benzoate

KW - Taste cell

KW - Whole-cell voltage-clamp

UR - http://www.scopus.com/inward/record.url?scp=0024326093&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024326093&partnerID=8YFLogxK

U2 - 10.1016/0006-8993(89)91684-3

DO - 10.1016/0006-8993(89)91684-3

M3 - Article

C2 - 2605526

AN - SCOPUS:0024326093

VL - 503

SP - 326

EP - 329

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 2

ER -