A Map of the Interactome Network of the Metazoan C. elegans

Siming Li, Christopher M. Armstrong, Nicolas Bertin, Hui Ge, Stuart Milstein, Mike Boxem, Pierre Olivier Vidalain, Jing Dong J Han, Alban Chesneau, Tong Hao, Debra S. Goldberg, Ning Li, Monica Martinez, Jean François Rual, Philippe Lamesch, Lai Xu, Muneesh Tewari, Sharyl L. Wong, Lan V. Zhang, Gabriel F. Berriz & 28 others Laurent Jacotot, Philippe Vaglio, Jérôme Reboul, Tomoko Hirozane-Kishikawa, Qianru Li, Harrison W. Gabel, Ahmed Elewa, Bridget Baumgartner, Debra J. Rose, Haiyuan Yu, Stephanie Bosak, Reynaldo Sequerra, Andrew Fraser, Susan E. Mango, William M. Saxton, Susan Strome, Sander v. Van Den Heuvel, Fabio Piano, Jean Vandenhaute, Claude Sardet, Mark Gerstein, Lynn Doucette-Stamm, Kristin C. Gunsalus, J. Wade Harper, Michael E. Cusick, Frederick P. Roth, David E. Hill, Marc Vidal

Research output: Contribution to journalArticle

Abstract

To initiate studies on how protein-protein interaction (or "interactome") networks relate to multicellular functions, we have mapped a large fraction of the Caenorhabditis elegans interactome network. Starting with a subset of metazoan-specific proteins, more than 4000 interactions were identified from high-throughput, yeast two-hybrid (HT=Y2H) screens. Independent coaffinity purification assays experimentally validated the overall quality of this Y2H data set. Together with already described Y2H interactions and interologs predicted in silico, the current version of the Worm Interactome (WI5) map contains ∼5500 interactions. Topological and biological features of this interactome network, as well as its integration with phenome and transcriptome data sets, lead to numerous biological hypotheses.

Original languageEnglish (US)
Pages (from-to)540-543
Number of pages4
JournalScience
Volume303
Issue number5657
DOIs
StatePublished - Jan 23 2004

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Proteins
Caenorhabditis elegans
Transcriptome
Computer Simulation
Yeasts
Datasets

ASJC Scopus subject areas

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Li, S., Armstrong, C. M., Bertin, N., Ge, H., Milstein, S., Boxem, M., ... Vidal, M. (2004). A Map of the Interactome Network of the Metazoan C. elegans. Science, 303(5657), 540-543. https://doi.org/10.1126/science.1091403

A Map of the Interactome Network of the Metazoan C. elegans. / Li, Siming; Armstrong, Christopher M.; Bertin, Nicolas; Ge, Hui; Milstein, Stuart; Boxem, Mike; Vidalain, Pierre Olivier; Han, Jing Dong J; Chesneau, Alban; Hao, Tong; Goldberg, Debra S.; Li, Ning; Martinez, Monica; Rual, Jean François; Lamesch, Philippe; Xu, Lai; Tewari, Muneesh; Wong, Sharyl L.; Zhang, Lan V.; Berriz, Gabriel F.; Jacotot, Laurent; Vaglio, Philippe; Reboul, Jérôme; Hirozane-Kishikawa, Tomoko; Li, Qianru; Gabel, Harrison W.; Elewa, Ahmed; Baumgartner, Bridget; Rose, Debra J.; Yu, Haiyuan; Bosak, Stephanie; Sequerra, Reynaldo; Fraser, Andrew; Mango, Susan E.; Saxton, William M.; Strome, Susan; Van Den Heuvel, Sander v.; Piano, Fabio; Vandenhaute, Jean; Sardet, Claude; Gerstein, Mark; Doucette-Stamm, Lynn; Gunsalus, Kristin C.; Harper, J. Wade; Cusick, Michael E.; Roth, Frederick P.; Hill, David E.; Vidal, Marc.

In: Science, Vol. 303, No. 5657, 23.01.2004, p. 540-543.

Research output: Contribution to journalArticle

Li, S, Armstrong, CM, Bertin, N, Ge, H, Milstein, S, Boxem, M, Vidalain, PO, Han, JDJ, Chesneau, A, Hao, T, Goldberg, DS, Li, N, Martinez, M, Rual, JF, Lamesch, P, Xu, L, Tewari, M, Wong, SL, Zhang, LV, Berriz, GF, Jacotot, L, Vaglio, P, Reboul, J, Hirozane-Kishikawa, T, Li, Q, Gabel, HW, Elewa, A, Baumgartner, B, Rose, DJ, Yu, H, Bosak, S, Sequerra, R, Fraser, A, Mango, SE, Saxton, WM, Strome, S, Van Den Heuvel, SV, Piano, F, Vandenhaute, J, Sardet, C, Gerstein, M, Doucette-Stamm, L, Gunsalus, KC, Harper, JW, Cusick, ME, Roth, FP, Hill, DE & Vidal, M 2004, 'A Map of the Interactome Network of the Metazoan C. elegans', Science, vol. 303, no. 5657, pp. 540-543. https://doi.org/10.1126/science.1091403
Li S, Armstrong CM, Bertin N, Ge H, Milstein S, Boxem M et al. A Map of the Interactome Network of the Metazoan C. elegans. Science. 2004 Jan 23;303(5657):540-543. https://doi.org/10.1126/science.1091403
Li, Siming ; Armstrong, Christopher M. ; Bertin, Nicolas ; Ge, Hui ; Milstein, Stuart ; Boxem, Mike ; Vidalain, Pierre Olivier ; Han, Jing Dong J ; Chesneau, Alban ; Hao, Tong ; Goldberg, Debra S. ; Li, Ning ; Martinez, Monica ; Rual, Jean François ; Lamesch, Philippe ; Xu, Lai ; Tewari, Muneesh ; Wong, Sharyl L. ; Zhang, Lan V. ; Berriz, Gabriel F. ; Jacotot, Laurent ; Vaglio, Philippe ; Reboul, Jérôme ; Hirozane-Kishikawa, Tomoko ; Li, Qianru ; Gabel, Harrison W. ; Elewa, Ahmed ; Baumgartner, Bridget ; Rose, Debra J. ; Yu, Haiyuan ; Bosak, Stephanie ; Sequerra, Reynaldo ; Fraser, Andrew ; Mango, Susan E. ; Saxton, William M. ; Strome, Susan ; Van Den Heuvel, Sander v. ; Piano, Fabio ; Vandenhaute, Jean ; Sardet, Claude ; Gerstein, Mark ; Doucette-Stamm, Lynn ; Gunsalus, Kristin C. ; Harper, J. Wade ; Cusick, Michael E. ; Roth, Frederick P. ; Hill, David E. ; Vidal, Marc. / A Map of the Interactome Network of the Metazoan C. elegans. In: Science. 2004 ; Vol. 303, No. 5657. pp. 540-543.
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AU - Li, Siming

AU - Armstrong, Christopher M.

AU - Bertin, Nicolas

AU - Ge, Hui

AU - Milstein, Stuart

AU - Boxem, Mike

AU - Vidalain, Pierre Olivier

AU - Han, Jing Dong J

AU - Chesneau, Alban

AU - Hao, Tong

AU - Goldberg, Debra S.

AU - Li, Ning

AU - Martinez, Monica

AU - Rual, Jean François

AU - Lamesch, Philippe

AU - Xu, Lai

AU - Tewari, Muneesh

AU - Wong, Sharyl L.

AU - Zhang, Lan V.

AU - Berriz, Gabriel F.

AU - Jacotot, Laurent

AU - Vaglio, Philippe

AU - Reboul, Jérôme

AU - Hirozane-Kishikawa, Tomoko

AU - Li, Qianru

AU - Gabel, Harrison W.

AU - Elewa, Ahmed

AU - Baumgartner, Bridget

AU - Rose, Debra J.

AU - Yu, Haiyuan

AU - Bosak, Stephanie

AU - Sequerra, Reynaldo

AU - Fraser, Andrew

AU - Mango, Susan E.

AU - Saxton, William M.

AU - Strome, Susan

AU - Van Den Heuvel, Sander v.

AU - Piano, Fabio

AU - Vandenhaute, Jean

AU - Sardet, Claude

AU - Gerstein, Mark

AU - Doucette-Stamm, Lynn

AU - Gunsalus, Kristin C.

AU - Harper, J. Wade

AU - Cusick, Michael E.

AU - Roth, Frederick P.

AU - Hill, David E.

AU - Vidal, Marc

PY - 2004/1/23

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