A map of cell type-specific auxin responses

Bastiaan O R Bargmann, Steffen Vanneste, Gabriel Krouk, Tal Nawy, Idan Efroni, Eilon Shani, Goh Choe, Jiří Friml, Dominique C. Bergmann, Mark Estelle, Kenneth D. Birnbaum

Research output: Contribution to journalArticle

Abstract

In plants, changes in local auxin concentrations can trigger a range of developmental processes as distinct tissues respond differently to the same auxin stimulus. However, little is known about how auxin is interpreted by individual cell types. We performed a transcriptomic analysis of responses to auxin within four distinct tissues of the Arabidopsis thaliana root and demonstrate that different cell types show competence for discrete responses. The majority of auxin-responsive genes displayed a spatial bias in their induction or repression. The novel data set was used to examine how auxin influences tissue-specific transcriptional regulation of cell-identity markers. Additionally, the data were used in combination with spatial expression maps of the root to plot a transcriptomic auxin-response gradient across the apical and basal meristem. The readout revealed a strong correlation for thousands of genes between the relative response to auxin and expression along the longitudinal axis of the root. This data set and comparative analysis provide a transcriptome-level spatial breakdown of the response to auxin within an organ where this hormone mediates many aspects of development.

Original languageEnglish (US)
Article number688
JournalMolecular systems biology [electronic resource].
Volume9
Issue number1
DOIs
StatePublished - 2013

Fingerprint

Indoleacetic Acids
Auxin
auxins
Tissue
Cell
Genes
Hormones
cells
transcriptomics
Cells
Roots
Gene
Distinct
Arabidopsis Thaliana
Transcriptional Regulation
Meristem
Transcriptome
Comparative Analysis
Arabidopsis
Trigger

Keywords

  • Arabidopsis
  • development
  • root apical meristem
  • signaling gradient

ASJC Scopus subject areas

  • Applied Mathematics
  • Information Systems
  • Computational Theory and Mathematics
  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Medicine(all)

Cite this

Bargmann, B. O. R., Vanneste, S., Krouk, G., Nawy, T., Efroni, I., Shani, E., ... Birnbaum, K. D. (2013). A map of cell type-specific auxin responses. Molecular systems biology [electronic resource]., 9(1), [688]. https://doi.org/10.1038/msb.2013.40

A map of cell type-specific auxin responses. / Bargmann, Bastiaan O R; Vanneste, Steffen; Krouk, Gabriel; Nawy, Tal; Efroni, Idan; Shani, Eilon; Choe, Goh; Friml, Jiří; Bergmann, Dominique C.; Estelle, Mark; Birnbaum, Kenneth D.

In: Molecular systems biology [electronic resource]., Vol. 9, No. 1, 688, 2013.

Research output: Contribution to journalArticle

Bargmann, BOR, Vanneste, S, Krouk, G, Nawy, T, Efroni, I, Shani, E, Choe, G, Friml, J, Bergmann, DC, Estelle, M & Birnbaum, KD 2013, 'A map of cell type-specific auxin responses', Molecular systems biology [electronic resource]., vol. 9, no. 1, 688. https://doi.org/10.1038/msb.2013.40
Bargmann BOR, Vanneste S, Krouk G, Nawy T, Efroni I, Shani E et al. A map of cell type-specific auxin responses. Molecular systems biology [electronic resource]. 2013;9(1). 688. https://doi.org/10.1038/msb.2013.40
Bargmann, Bastiaan O R ; Vanneste, Steffen ; Krouk, Gabriel ; Nawy, Tal ; Efroni, Idan ; Shani, Eilon ; Choe, Goh ; Friml, Jiří ; Bergmann, Dominique C. ; Estelle, Mark ; Birnbaum, Kenneth D. / A map of cell type-specific auxin responses. In: Molecular systems biology [electronic resource]. 2013 ; Vol. 9, No. 1.
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