A large-scale screen reveals genes that mediate electrotaxis in Dictyostelium discoideum

Runchi Gao, Siwei Zhao, Xupin Jiang, Yaohui Sun, Sanjun Zhao, Jing Gao, Jane Borleis, Stacey Willard, Ming Tang, Huaqing Cai, Yoichiro Kamimura, Yuesheng Huang, Jianxin Jiang, Zunxi Huang, Alex Mogilner, Tingrui Pan, Peter N. Devreotes, Min Zhao

Research output: Contribution to journalArticle

Abstract

Directional cell migration in an electric field, a phenomenon called galvanotaxis or electrotaxis, occurs in many types of cells, and may play an important role in wound healing and development. Small extracellular electric fields can guide the migration of amoeboid cells, and we established a large-scale screening approach to search for mutants with electrotaxis phenotypes from a collection of 563 Dictyostelium discoideum strains with morphological defects. We identified 28 strains that were defective in electrotaxis and 10 strains with a slightly higher directional response. Using plasmid rescue followed by gene disruption, we identified some of the mutated genes, including some previously implicated in chemotaxis. Among these, we studied PiaA, which encodes a critical component of TORC2, a kinase protein complex that transduces changes in motility by activating the kinase PKB (also known as Akt). Furthermore, we found that electrotaxis was decreased in mutants lacking gefA, rasC, rip3, lst8, or pkbR1, genes that encode other components of the TORC2-PKB pathway. Thus, we have developed a high-throughput screening technique that will be a useful tool to elucidate the molecular mechanisms of electrotaxis.

Original languageEnglish (US)
JournalScience Signaling
Volume8
Issue number378
DOIs
StatePublished - May 26 2015

Fingerprint

Dictyostelium
Genes
Cell Movement
Screening
Electric fields
Chemotaxis
Wound Healing
Protein Kinases
Plasmids
Phosphotransferases
Cells
Throughput
Phenotype
Defects
TOR complex 2

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

A large-scale screen reveals genes that mediate electrotaxis in Dictyostelium discoideum. / Gao, Runchi; Zhao, Siwei; Jiang, Xupin; Sun, Yaohui; Zhao, Sanjun; Gao, Jing; Borleis, Jane; Willard, Stacey; Tang, Ming; Cai, Huaqing; Kamimura, Yoichiro; Huang, Yuesheng; Jiang, Jianxin; Huang, Zunxi; Mogilner, Alex; Pan, Tingrui; Devreotes, Peter N.; Zhao, Min.

In: Science Signaling, Vol. 8, No. 378, 26.05.2015.

Research output: Contribution to journalArticle

Gao, R, Zhao, S, Jiang, X, Sun, Y, Zhao, S, Gao, J, Borleis, J, Willard, S, Tang, M, Cai, H, Kamimura, Y, Huang, Y, Jiang, J, Huang, Z, Mogilner, A, Pan, T, Devreotes, PN & Zhao, M 2015, 'A large-scale screen reveals genes that mediate electrotaxis in Dictyostelium discoideum', Science Signaling, vol. 8, no. 378. https://doi.org/10.1126/scisignal.aab0562
Gao, Runchi ; Zhao, Siwei ; Jiang, Xupin ; Sun, Yaohui ; Zhao, Sanjun ; Gao, Jing ; Borleis, Jane ; Willard, Stacey ; Tang, Ming ; Cai, Huaqing ; Kamimura, Yoichiro ; Huang, Yuesheng ; Jiang, Jianxin ; Huang, Zunxi ; Mogilner, Alex ; Pan, Tingrui ; Devreotes, Peter N. ; Zhao, Min. / A large-scale screen reveals genes that mediate electrotaxis in Dictyostelium discoideum. In: Science Signaling. 2015 ; Vol. 8, No. 378.
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