A high resolution 1H NMR study of the solution structure of human epidermal growth factor

J. A. Carver, R. M. Cooke, Gennaro Esposito, I. D. Campbell, H. Gregory, B. Sheard

    Research output: Contribution to journalArticle

    Abstract

    500 MHz 1H NMR studies of human epidermal growth factor are described. The backbone resonances of the 1-48 derivative of hEGF have been assigned using two-dimensional techniques. Analysis of the type and magnitude of the observed sequential nuclear Overhauser effects and the NH-αCH spin-spin coupling constants allowed prediction of the secondary structure. Aspects of the tertiary structure are also identified. A pair of antiparallel β-sheets involving residues 18-23 and 28-34 is a dominant feature of the solution structure.

    Original languageEnglish (US)
    Pages (from-to)77-81
    Number of pages5
    JournalFEBS Letters
    Volume205
    Issue number1
    DOIs
    StatePublished - Sep 1 1986

    Fingerprint

    Epidermal Growth Factor
    Nuclear magnetic resonance
    Derivatives
    epidermal growth factor (1-48)
    Proton Magnetic Resonance Spectroscopy

    Keywords

    • (Human EGF)
    • Growth factor
    • NMR
    • Protein structure

    ASJC Scopus subject areas

    • Biophysics
    • Structural Biology
    • Biochemistry
    • Medicine(all)
    • Molecular Biology
    • Genetics
    • Cell Biology

    Cite this

    Carver, J. A., Cooke, R. M., Esposito, G., Campbell, I. D., Gregory, H., & Sheard, B. (1986). A high resolution 1H NMR study of the solution structure of human epidermal growth factor. FEBS Letters, 205(1), 77-81. https://doi.org/10.1016/0014-5793(86)80869-9

    A high resolution 1H NMR study of the solution structure of human epidermal growth factor. / Carver, J. A.; Cooke, R. M.; Esposito, Gennaro; Campbell, I. D.; Gregory, H.; Sheard, B.

    In: FEBS Letters, Vol. 205, No. 1, 01.09.1986, p. 77-81.

    Research output: Contribution to journalArticle

    Carver, JA, Cooke, RM, Esposito, G, Campbell, ID, Gregory, H & Sheard, B 1986, 'A high resolution 1H NMR study of the solution structure of human epidermal growth factor', FEBS Letters, vol. 205, no. 1, pp. 77-81. https://doi.org/10.1016/0014-5793(86)80869-9
    Carver, J. A. ; Cooke, R. M. ; Esposito, Gennaro ; Campbell, I. D. ; Gregory, H. ; Sheard, B. / A high resolution 1H NMR study of the solution structure of human epidermal growth factor. In: FEBS Letters. 1986 ; Vol. 205, No. 1. pp. 77-81.
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