Abstract
The nonrandom distribution of meiotic recombination influences patterns of inheritance and genome evolution, but chromosomal features governing this distribution are poorly understood. Formation of the DNA double-strand breaks (DSBs) that initiate recombination results in the accumulation of Spo11 protein covalently bound to small DNA fragments. By sequencing these fragments, we uncover a genome-wide DSB map of unprecedented resolution and sensitivity. We use this map to explore how DSB distribution is influenced by large-scale chromosome structures, chromatin, transcription factors, and local sequence composition. Our analysis offers mechanistic insight into DSB formation and early processing steps, supporting the view that the recombination terrain is molded by combinatorial and hierarchical interaction of factors that work on widely different size scales. This map illuminates the occurrence of DSBs in repetitive DNA elements, repair of which can lead to chromosomal rearrangements. We also discuss implications for evolutionary dynamics of recombination hot spots.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 719-731 |
| Number of pages | 13 |
| Journal | Cell |
| Volume | 144 |
| Issue number | 5 |
| DOIs | |
| State | Published - Mar 4 2011 |
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ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
A hierarchical combination of factors shapes the genome-wide topography of yeast meiotic recombination initiation. / Pan, Jing; Sasaki, Mariko; Kniewel, Ryan; Murakami, Hajime; Blitzblau, Hannah G.; Tischfield, Sam E.; Zhu, Xuan; Neale, Matthew J.; Jasin, Maria; Socci, Nicholas D.; Hochwagen, Andreas; Keeney, Scott.
In: Cell, Vol. 144, No. 5, 04.03.2011, p. 719-731.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A hierarchical combination of factors shapes the genome-wide topography of yeast meiotic recombination initiation
AU - Pan, Jing
AU - Sasaki, Mariko
AU - Kniewel, Ryan
AU - Murakami, Hajime
AU - Blitzblau, Hannah G.
AU - Tischfield, Sam E.
AU - Zhu, Xuan
AU - Neale, Matthew J.
AU - Jasin, Maria
AU - Socci, Nicholas D.
AU - Hochwagen, Andreas
AU - Keeney, Scott
PY - 2011/3/4
Y1 - 2011/3/4
N2 - The nonrandom distribution of meiotic recombination influences patterns of inheritance and genome evolution, but chromosomal features governing this distribution are poorly understood. Formation of the DNA double-strand breaks (DSBs) that initiate recombination results in the accumulation of Spo11 protein covalently bound to small DNA fragments. By sequencing these fragments, we uncover a genome-wide DSB map of unprecedented resolution and sensitivity. We use this map to explore how DSB distribution is influenced by large-scale chromosome structures, chromatin, transcription factors, and local sequence composition. Our analysis offers mechanistic insight into DSB formation and early processing steps, supporting the view that the recombination terrain is molded by combinatorial and hierarchical interaction of factors that work on widely different size scales. This map illuminates the occurrence of DSBs in repetitive DNA elements, repair of which can lead to chromosomal rearrangements. We also discuss implications for evolutionary dynamics of recombination hot spots.
AB - The nonrandom distribution of meiotic recombination influences patterns of inheritance and genome evolution, but chromosomal features governing this distribution are poorly understood. Formation of the DNA double-strand breaks (DSBs) that initiate recombination results in the accumulation of Spo11 protein covalently bound to small DNA fragments. By sequencing these fragments, we uncover a genome-wide DSB map of unprecedented resolution and sensitivity. We use this map to explore how DSB distribution is influenced by large-scale chromosome structures, chromatin, transcription factors, and local sequence composition. Our analysis offers mechanistic insight into DSB formation and early processing steps, supporting the view that the recombination terrain is molded by combinatorial and hierarchical interaction of factors that work on widely different size scales. This map illuminates the occurrence of DSBs in repetitive DNA elements, repair of which can lead to chromosomal rearrangements. We also discuss implications for evolutionary dynamics of recombination hot spots.
UR - http://www.scopus.com/inward/record.url?scp=79952295560&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79952295560&partnerID=8YFLogxK
U2 - 10.1016/j.cell.2011.02.009
DO - 10.1016/j.cell.2011.02.009
M3 - Article
C2 - 21376234
AN - SCOPUS:79952295560
VL - 144
SP - 719
EP - 731
JO - Cell
JF - Cell
SN - 0092-8674
IS - 5
ER -