A hierarchical combination of factors shapes the genome-wide topography of yeast meiotic recombination initiation

Jing Pan, Mariko Sasaki, Ryan Kniewel, Hajime Murakami, Hannah G. Blitzblau, Sam E. Tischfield, Xuan Zhu, Matthew J. Neale, Maria Jasin, Nicholas D. Socci, Andreas Hochwagen, Scott Keeney

Research output: Contribution to journalArticle

Abstract

The nonrandom distribution of meiotic recombination influences patterns of inheritance and genome evolution, but chromosomal features governing this distribution are poorly understood. Formation of the DNA double-strand breaks (DSBs) that initiate recombination results in the accumulation of Spo11 protein covalently bound to small DNA fragments. By sequencing these fragments, we uncover a genome-wide DSB map of unprecedented resolution and sensitivity. We use this map to explore how DSB distribution is influenced by large-scale chromosome structures, chromatin, transcription factors, and local sequence composition. Our analysis offers mechanistic insight into DSB formation and early processing steps, supporting the view that the recombination terrain is molded by combinatorial and hierarchical interaction of factors that work on widely different size scales. This map illuminates the occurrence of DSBs in repetitive DNA elements, repair of which can lead to chromosomal rearrangements. We also discuss implications for evolutionary dynamics of recombination hot spots.

Original languageEnglish (US)
Pages (from-to)719-731
Number of pages13
JournalCell
Volume144
Issue number5
DOIs
StatePublished - Mar 4 2011

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Yeast
Topography
Genetic Recombination
Genes
Yeasts
Genome
DNA
Chromosomes
Chromosome Structures
Inheritance Patterns
Chromatin
Double-Stranded DNA Breaks
Repair
Transcription Factors
DNA Repair
Processing
Chemical analysis
Proteins

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Pan, J., Sasaki, M., Kniewel, R., Murakami, H., Blitzblau, H. G., Tischfield, S. E., ... Keeney, S. (2011). A hierarchical combination of factors shapes the genome-wide topography of yeast meiotic recombination initiation. Cell, 144(5), 719-731. https://doi.org/10.1016/j.cell.2011.02.009

A hierarchical combination of factors shapes the genome-wide topography of yeast meiotic recombination initiation. / Pan, Jing; Sasaki, Mariko; Kniewel, Ryan; Murakami, Hajime; Blitzblau, Hannah G.; Tischfield, Sam E.; Zhu, Xuan; Neale, Matthew J.; Jasin, Maria; Socci, Nicholas D.; Hochwagen, Andreas; Keeney, Scott.

In: Cell, Vol. 144, No. 5, 04.03.2011, p. 719-731.

Research output: Contribution to journalArticle

Pan, J, Sasaki, M, Kniewel, R, Murakami, H, Blitzblau, HG, Tischfield, SE, Zhu, X, Neale, MJ, Jasin, M, Socci, ND, Hochwagen, A & Keeney, S 2011, 'A hierarchical combination of factors shapes the genome-wide topography of yeast meiotic recombination initiation', Cell, vol. 144, no. 5, pp. 719-731. https://doi.org/10.1016/j.cell.2011.02.009
Pan J, Sasaki M, Kniewel R, Murakami H, Blitzblau HG, Tischfield SE et al. A hierarchical combination of factors shapes the genome-wide topography of yeast meiotic recombination initiation. Cell. 2011 Mar 4;144(5):719-731. https://doi.org/10.1016/j.cell.2011.02.009
Pan, Jing ; Sasaki, Mariko ; Kniewel, Ryan ; Murakami, Hajime ; Blitzblau, Hannah G. ; Tischfield, Sam E. ; Zhu, Xuan ; Neale, Matthew J. ; Jasin, Maria ; Socci, Nicholas D. ; Hochwagen, Andreas ; Keeney, Scott. / A hierarchical combination of factors shapes the genome-wide topography of yeast meiotic recombination initiation. In: Cell. 2011 ; Vol. 144, No. 5. pp. 719-731.
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