A disposable microfluidic point-of-care device for the detection of HIV: A new up-converting phosphor technology application

Z. Chen, P. L A M Corstjens, M. Zuiderwijk, J. Wang, M. G. Mauk, H. H. Bau, W. R. Abrams, Daniel Malamud

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A bench-top analysis protocol to detect HIV-1 antibodies in blood plasma with upconverting phosphor particles (UPT) and lateral flow (LF) was established. A preliminary clinical study demonstrated that the UPT-LF detection successfully identified positive and negative samples. A 104× dilution of a positive clinical sample generated a signal well above the negative control. The bench-top UPT-LF protocol was implemented in a microfluidic chip with functions such as sample introduction, buffer distribution, metering, mixing, and thermo -pneumatic pump ing. The flow control was achieved with phase-change (PC) valves that operated in a self-actuated mode. Preliminary tests of the microfluidic device demonstrated compatibility of the microfluidic chip with UPT-LF detection and produced comparable sensitivity to that of the bench-top test.

Original languageEnglish (US)
Title of host publicationMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherTransducer Research Foundation
Pages791-793
Number of pages3
Volume1
ISBN (Print)0974361119, 9780974361116
StatePublished - 2005
Event9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005 - Boston, MA, United States
Duration: Oct 9 2005Oct 13 2005

Other

Other9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005
CountryUnited States
CityBoston, MA
Period10/9/0510/13/05

Fingerprint

Microfluidics
Phosphors
Flow control
Antibodies
Pneumatics
Dilution
Buffers
Blood
Pumps
Plasmas

Keywords

  • HIV detection
  • Immunoassay
  • Lab-on-a-chip
  • Phase-change valve

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Chen, Z., Corstjens, P. L. A. M., Zuiderwijk, M., Wang, J., Mauk, M. G., Bau, H. H., ... Malamud, D. (2005). A disposable microfluidic point-of-care device for the detection of HIV: A new up-converting phosphor technology application. In Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences (Vol. 1, pp. 791-793). Transducer Research Foundation.

A disposable microfluidic point-of-care device for the detection of HIV : A new up-converting phosphor technology application. / Chen, Z.; Corstjens, P. L A M; Zuiderwijk, M.; Wang, J.; Mauk, M. G.; Bau, H. H.; Abrams, W. R.; Malamud, Daniel.

Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Vol. 1 Transducer Research Foundation, 2005. p. 791-793.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Chen, Z, Corstjens, PLAM, Zuiderwijk, M, Wang, J, Mauk, MG, Bau, HH, Abrams, WR & Malamud, D 2005, A disposable microfluidic point-of-care device for the detection of HIV: A new up-converting phosphor technology application. in Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences. vol. 1, Transducer Research Foundation, pp. 791-793, 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005, Boston, MA, United States, 10/9/05.
Chen Z, Corstjens PLAM, Zuiderwijk M, Wang J, Mauk MG, Bau HH et al. A disposable microfluidic point-of-care device for the detection of HIV: A new up-converting phosphor technology application. In Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Vol. 1. Transducer Research Foundation. 2005. p. 791-793
Chen, Z. ; Corstjens, P. L A M ; Zuiderwijk, M. ; Wang, J. ; Mauk, M. G. ; Bau, H. H. ; Abrams, W. R. ; Malamud, Daniel. / A disposable microfluidic point-of-care device for the detection of HIV : A new up-converting phosphor technology application. Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Vol. 1 Transducer Research Foundation, 2005. pp. 791-793
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