A critical assessment of the utility of protein-free splicing systems

Duncan Smith, Maria M. Konarska

Research output: Contribution to journalArticle

Abstract

U2 and U6 snRNAs form part of the catalytic spliceosome and represent strong candidates for components of its active site. Over the past decade it has become clear that these snRNAs are capable of catalyzing several different chemical reactions, leading to the widespread conclusion that the spliceosome is a ribozyme. Here, we discuss the advances in both protein-free and fully spliceosomal systems that would be required to conclude that the reactions observed to be catalyzed by protein-free snRNAs are related to splicing and question the reliability of snRNA-only systems as tools for mechanistic splicing research. Published by Cold Spring Harbor Laboratory Press.

Original languageEnglish (US)
Pages (from-to)1-3
Number of pages3
JournalRNA
Volume15
Issue number1
DOIs
StatePublished - Jan 2009

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Protein Splicing
Spliceosomes
Small Nuclear RNA
Catalytic RNA
Catalytic Domain
Proteins
Research

Keywords

  • RNA catalysis
  • snRNAs
  • Splicing

ASJC Scopus subject areas

  • Molecular Biology

Cite this

A critical assessment of the utility of protein-free splicing systems. / Smith, Duncan; Konarska, Maria M.

In: RNA, Vol. 15, No. 1, 01.2009, p. 1-3.

Research output: Contribution to journalArticle

Smith, Duncan ; Konarska, Maria M. / A critical assessment of the utility of protein-free splicing systems. In: RNA. 2009 ; Vol. 15, No. 1. pp. 1-3.
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