A cosmid vector containing a dominant selectable marker for cloning Chlamydomonas genes by complementation

Mary Chang, Fei Li, O. W. Odom, Jaesung Lee, David L. Herrin

Research output: Contribution to journalArticle

Abstract

Cosmid vectors containing a dominate selectable marker (ble) for complementation cloning of genes in Chlamydomonas reinhardtii were created. The usefulness of these vectors, which differ in the orientation of the ble cassette, was demonstrated by transforming C. reinhardtii to phleomycin resistance, by constructing a large library (∼5×105 recombinants) in one of them using DNA from a C. reinhardtii mutant, and by transforming C. reinhardtii with recombinant cosmid clones and pools.

Original languageEnglish (US)
Pages (from-to)75-78
Number of pages4
JournalPlasmid
Volume49
Issue number1
DOIs
StatePublished - Jan 2003

Fingerprint

Chlamydomonas
Chlamydomonas reinhardtii
Cosmids
Organism Cloning
Genes
Phleomycins
Libraries
Clone Cells
DNA

Keywords

  • ble cassette
  • Chlamydomonas
  • Complementation cloning
  • Cosmid vector
  • Dominant marker
  • Phleomycin resistance

ASJC Scopus subject areas

  • Molecular Biology

Cite this

A cosmid vector containing a dominant selectable marker for cloning Chlamydomonas genes by complementation. / Chang, Mary; Li, Fei; Odom, O. W.; Lee, Jaesung; Herrin, David L.

In: Plasmid, Vol. 49, No. 1, 01.2003, p. 75-78.

Research output: Contribution to journalArticle

Chang, Mary ; Li, Fei ; Odom, O. W. ; Lee, Jaesung ; Herrin, David L. / A cosmid vector containing a dominant selectable marker for cloning Chlamydomonas genes by complementation. In: Plasmid. 2003 ; Vol. 49, No. 1. pp. 75-78.
@article{51934e2b623c4767b1360aec14cb66dc,
title = "A cosmid vector containing a dominant selectable marker for cloning Chlamydomonas genes by complementation",
abstract = "Cosmid vectors containing a dominate selectable marker (ble) for complementation cloning of genes in Chlamydomonas reinhardtii were created. The usefulness of these vectors, which differ in the orientation of the ble cassette, was demonstrated by transforming C. reinhardtii to phleomycin resistance, by constructing a large library (∼5×105 recombinants) in one of them using DNA from a C. reinhardtii mutant, and by transforming C. reinhardtii with recombinant cosmid clones and pools.",
keywords = "ble cassette, Chlamydomonas, Complementation cloning, Cosmid vector, Dominant marker, Phleomycin resistance",
author = "Mary Chang and Fei Li and Odom, {O. W.} and Jaesung Lee and Herrin, {David L.}",
year = "2003",
month = "1",
doi = "10.1016/S0147-619X(02)00158-0",
language = "English (US)",
volume = "49",
pages = "75--78",
journal = "Plasmid",
issn = "0147-619X",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - A cosmid vector containing a dominant selectable marker for cloning Chlamydomonas genes by complementation

AU - Chang, Mary

AU - Li, Fei

AU - Odom, O. W.

AU - Lee, Jaesung

AU - Herrin, David L.

PY - 2003/1

Y1 - 2003/1

N2 - Cosmid vectors containing a dominate selectable marker (ble) for complementation cloning of genes in Chlamydomonas reinhardtii were created. The usefulness of these vectors, which differ in the orientation of the ble cassette, was demonstrated by transforming C. reinhardtii to phleomycin resistance, by constructing a large library (∼5×105 recombinants) in one of them using DNA from a C. reinhardtii mutant, and by transforming C. reinhardtii with recombinant cosmid clones and pools.

AB - Cosmid vectors containing a dominate selectable marker (ble) for complementation cloning of genes in Chlamydomonas reinhardtii were created. The usefulness of these vectors, which differ in the orientation of the ble cassette, was demonstrated by transforming C. reinhardtii to phleomycin resistance, by constructing a large library (∼5×105 recombinants) in one of them using DNA from a C. reinhardtii mutant, and by transforming C. reinhardtii with recombinant cosmid clones and pools.

KW - ble cassette

KW - Chlamydomonas

KW - Complementation cloning

KW - Cosmid vector

KW - Dominant marker

KW - Phleomycin resistance

UR - http://www.scopus.com/inward/record.url?scp=0037287943&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037287943&partnerID=8YFLogxK

U2 - 10.1016/S0147-619X(02)00158-0

DO - 10.1016/S0147-619X(02)00158-0

M3 - Article

VL - 49

SP - 75

EP - 78

JO - Plasmid

JF - Plasmid

SN - 0147-619X

IS - 1

ER -