A comprehensive survey of the Plasmodium life cycle by genomic, transcriptomic, and proteomic analyses

Neil Hall, Marianna Karras, J. Dale Raine, Jane M. Carlton, Taco W A Kooij, Matthew Berriman, Laurence Florens, Christoph S. Janssen, Arnab Pain, Georges K. Christophides, Keith James, Kim Rutherford, Barbara Harris, David Harris, Carol Churcher, Michael A. Quail, Doug Ormond, Jon Doggett, Holly E. Trueman, Jacqui MendozaShelby L. Bidwell, Marie Adele Rajandream, Daniel J. Carucci, John R. Yates, Fotis C. Kafatos, Chris J. Janse, Bart Barrell, C. Michael R Turner, Andrew P. Waters, Robert E. Sinden

Research output: Contribution to journalArticle

Abstract

Plasmodium berghei and Plasmodium chabaudi are widely used model malaria species. Comparison of their genomes, integrated with proteomic and microarray data, with the genomes of Plasmodium falciparum and Plasmodium yoelii revealed a conserved core of 4500 Plasmodium genes in the central regions of the 14 chromosomes and highlighted genes evolving rapidly because of stage-specific selective pressures. Four strategies for gene expression are apparent during the parasites' life cycle: (i) housekeeping; (ii) host-related; (iii) strategy-specific related to invasion, asexual replication, and sexual development; and (iv) stage-specific. We observed posttranscriptional gene silencing through translational repression of messenger RNA during sexual development, and a 47-base 3′ untranslated region motif is implicated in this process.

Original languageEnglish (US)
Pages (from-to)82-86
Number of pages5
JournalScience
Volume307
Issue number5706
DOIs
StatePublished - Jan 7 2005

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Sexual Development
Plasmodium
Life Cycle Stages
Proteomics
Plasmodium chabaudi
Plasmodium yoelii
Genome
Chromosomes, Human, Pair 14
Plasmodium berghei
Housekeeping
3' Untranslated Regions
Plasmodium falciparum
RNA Interference
Genes
Malaria
Parasites
Gene Expression
Messenger RNA
Surveys and Questionnaires

ASJC Scopus subject areas

  • General

Cite this

Hall, N., Karras, M., Raine, J. D., Carlton, J. M., Kooij, T. W. A., Berriman, M., ... Sinden, R. E. (2005). A comprehensive survey of the Plasmodium life cycle by genomic, transcriptomic, and proteomic analyses. Science, 307(5706), 82-86. https://doi.org/10.1126/science.1103717

A comprehensive survey of the Plasmodium life cycle by genomic, transcriptomic, and proteomic analyses. / Hall, Neil; Karras, Marianna; Raine, J. Dale; Carlton, Jane M.; Kooij, Taco W A; Berriman, Matthew; Florens, Laurence; Janssen, Christoph S.; Pain, Arnab; Christophides, Georges K.; James, Keith; Rutherford, Kim; Harris, Barbara; Harris, David; Churcher, Carol; Quail, Michael A.; Ormond, Doug; Doggett, Jon; Trueman, Holly E.; Mendoza, Jacqui; Bidwell, Shelby L.; Rajandream, Marie Adele; Carucci, Daniel J.; Yates, John R.; Kafatos, Fotis C.; Janse, Chris J.; Barrell, Bart; Turner, C. Michael R; Waters, Andrew P.; Sinden, Robert E.

In: Science, Vol. 307, No. 5706, 07.01.2005, p. 82-86.

Research output: Contribution to journalArticle

Hall, N, Karras, M, Raine, JD, Carlton, JM, Kooij, TWA, Berriman, M, Florens, L, Janssen, CS, Pain, A, Christophides, GK, James, K, Rutherford, K, Harris, B, Harris, D, Churcher, C, Quail, MA, Ormond, D, Doggett, J, Trueman, HE, Mendoza, J, Bidwell, SL, Rajandream, MA, Carucci, DJ, Yates, JR, Kafatos, FC, Janse, CJ, Barrell, B, Turner, CMR, Waters, AP & Sinden, RE 2005, 'A comprehensive survey of the Plasmodium life cycle by genomic, transcriptomic, and proteomic analyses', Science, vol. 307, no. 5706, pp. 82-86. https://doi.org/10.1126/science.1103717
Hall, Neil ; Karras, Marianna ; Raine, J. Dale ; Carlton, Jane M. ; Kooij, Taco W A ; Berriman, Matthew ; Florens, Laurence ; Janssen, Christoph S. ; Pain, Arnab ; Christophides, Georges K. ; James, Keith ; Rutherford, Kim ; Harris, Barbara ; Harris, David ; Churcher, Carol ; Quail, Michael A. ; Ormond, Doug ; Doggett, Jon ; Trueman, Holly E. ; Mendoza, Jacqui ; Bidwell, Shelby L. ; Rajandream, Marie Adele ; Carucci, Daniel J. ; Yates, John R. ; Kafatos, Fotis C. ; Janse, Chris J. ; Barrell, Bart ; Turner, C. Michael R ; Waters, Andrew P. ; Sinden, Robert E. / A comprehensive survey of the Plasmodium life cycle by genomic, transcriptomic, and proteomic analyses. In: Science. 2005 ; Vol. 307, No. 5706. pp. 82-86.
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AU - Yates, John R.

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