A comparison of MTA, Super-EBA, composite and amalgam as root-end filling materials using a bacterial microleakage model

H. L. Adamo, R. Buruiana, L. Schertzer, Robert Boylan

Research output: Contribution to journalArticle

Abstract

Aim: The aim of this study was to compare traditional and newly developed root-end filling materials for resistance to bacterial microleakage. Methodology: Sixty extracted single-rooted teeth were randomly divided into five groups for root-end filling with mineral trioxide aggregate, Super-EBA, TPH composite resin with ProBond dentine bonding agent, Dispersalloy amalgam with and without ProBond, and positive and negative control groups. Root canals were instrumented using the step-back technique and simulated root-end resections performed. Root-end filling materials were placed in 3 mm ultrasonic retropreparations. Nail varnish was applied to all external root surfaces to the level of the resected root ends to prevent lateral microleakage. Samples were sterilized in an ethylene oxide sterilizer for 12 h. Using a newly designed model system, the apical 3-4 mm of the roots were immersed in BHI culture medium with phenol red indicator within culture chambers. The coronal access of each specimen was inoculated every 48 h with a suspension of Streptococcus salivarius. Culture media were observed every 24 h for colour change indicating bacterial contamination. Media demonstrating colour change were plated for S. salivarius. Samples were observed for 12 weeks. Results: At 4 weeks 10% of specimens from each experimental group had evidence of leakage. At 8 weeks 20% of specimens filled with amalgam without dentine bonding agent, Super-EBA and MTA had evidence of leakage. At 12 weeks minor differences between materials were noted. Conclusions: Under the conditions of the study, despite some variations, there were no statistically significant differences in rate of microleakage among the five groups tested at either 4, 8 or 12 weeks.

Original languageEnglish (US)
Pages (from-to)197-203
Number of pages7
JournalInternational Endodontic Journal
Volume32
Issue number3
DOIs
StatePublished - May 1999

Fingerprint

Pemetrexed
Dentin-Bonding Agents
Culture Media
Color
Phenolsulfonphthalein
Ethylene Oxide
Paint
Composite Resins
Dental Pulp Cavity
Nails
Ultrasonics
Suspensions
Tooth
Control Groups
Super EBA
Streptococcus salivarius

Keywords

  • Bacterial microleakage
  • Microleakage
  • Root-end fillings

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

A comparison of MTA, Super-EBA, composite and amalgam as root-end filling materials using a bacterial microleakage model. / Adamo, H. L.; Buruiana, R.; Schertzer, L.; Boylan, Robert.

In: International Endodontic Journal, Vol. 32, No. 3, 05.1999, p. 197-203.

Research output: Contribution to journalArticle

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abstract = "Aim: The aim of this study was to compare traditional and newly developed root-end filling materials for resistance to bacterial microleakage. Methodology: Sixty extracted single-rooted teeth were randomly divided into five groups for root-end filling with mineral trioxide aggregate, Super-EBA, TPH composite resin with ProBond dentine bonding agent, Dispersalloy amalgam with and without ProBond, and positive and negative control groups. Root canals were instrumented using the step-back technique and simulated root-end resections performed. Root-end filling materials were placed in 3 mm ultrasonic retropreparations. Nail varnish was applied to all external root surfaces to the level of the resected root ends to prevent lateral microleakage. Samples were sterilized in an ethylene oxide sterilizer for 12 h. Using a newly designed model system, the apical 3-4 mm of the roots were immersed in BHI culture medium with phenol red indicator within culture chambers. The coronal access of each specimen was inoculated every 48 h with a suspension of Streptococcus salivarius. Culture media were observed every 24 h for colour change indicating bacterial contamination. Media demonstrating colour change were plated for S. salivarius. Samples were observed for 12 weeks. Results: At 4 weeks 10{\%} of specimens from each experimental group had evidence of leakage. At 8 weeks 20{\%} of specimens filled with amalgam without dentine bonding agent, Super-EBA and MTA had evidence of leakage. At 12 weeks minor differences between materials were noted. Conclusions: Under the conditions of the study, despite some variations, there were no statistically significant differences in rate of microleakage among the five groups tested at either 4, 8 or 12 weeks.",
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