A collagen membrane containing osteogenic protein-1 facilitates bone regeneration in a rat mandibular bone defect

Manami Ozaki, Tadahiro Takayama, Takanobu Yamamoto, Yasumasa Ozawa, Mayu Nagao, Natsuko Tanabe, Akira Nakajima, Naoto Suzuki, Masao Maeno, Seiichi Yamano, Shuichi Sato

Research output: Contribution to journalArticle

Abstract

Objectives Osteogenic protein-1 (OP-1) has shown osteoinductive activities and is useful for clinical treatments, including bone regeneration. Regenerative procedures using a bioabsorbable collagen membrane (BCM) are well established in periodontal and implant dentistry. We evaluated the subsequent effects of the BCM in combination with OP-1 on bone regeneration in a rat mandibular circular critical-sized bone defect in vivo. Design We used 8 rats that received surgery in both sides of the mandible, and created the total 16 defects which were divided into 4 groups: Group 1; no treatment, as a control, Group 2; BCM alone, Group 3; BCM containing low dose 0.5 μg of OP-1 (L-OP-1), and Group 4; BCM containing high dose 2.0 μg of OP-1 (H-OP-1). Newly formed bone was evaluated by micro computed tomography (micro-CT) and histological analyses at 8 weeks postoperatively. In quantitative and qualitative micro-CT analyses of the volume of new bone formation, bone density, and percentage of new bone area was evaluated. Results BCM with rhOP-1 significantly increased and accelerated bone volume, bone mineral density, and percentage of new bone area compared to control and BCM alone at 8 weeks after surgery; these enhancements in bone regeneration in the OP-1-treated groups were dose-dependent. Conclusions OP-1 delivered with a BCM may have effective osteoinductive potency and be a good combination for bone regeneration. The use of such a combination device for osteogenesis may result in safer and more predictable bone regenerative outcomes in the future.

Original languageEnglish (US)
Pages (from-to)19-28
Number of pages10
JournalArchives of Oral Biology
Volume84
DOIs
StatePublished - Dec 1 2017

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Bone Morphogenetic Protein 7
Bone Regeneration
Collagen
Bone and Bones
Membranes
Osteogenesis
Bone Density
Cone-Beam Computed Tomography
Dentistry
Mandible
Tomography
Equipment and Supplies
Control Groups

Keywords

  • Bone regeneration
  • Collagen membrane
  • Micro-CT analysis
  • Osteogenesis
  • Osteogenic protein-1

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Dentistry(all)
  • Cell Biology

Cite this

A collagen membrane containing osteogenic protein-1 facilitates bone regeneration in a rat mandibular bone defect. / Ozaki, Manami; Takayama, Tadahiro; Yamamoto, Takanobu; Ozawa, Yasumasa; Nagao, Mayu; Tanabe, Natsuko; Nakajima, Akira; Suzuki, Naoto; Maeno, Masao; Yamano, Seiichi; Sato, Shuichi.

In: Archives of Oral Biology, Vol. 84, 01.12.2017, p. 19-28.

Research output: Contribution to journalArticle

Ozaki, M, Takayama, T, Yamamoto, T, Ozawa, Y, Nagao, M, Tanabe, N, Nakajima, A, Suzuki, N, Maeno, M, Yamano, S & Sato, S 2017, 'A collagen membrane containing osteogenic protein-1 facilitates bone regeneration in a rat mandibular bone defect', Archives of Oral Biology, vol. 84, pp. 19-28. https://doi.org/10.1016/j.archoralbio.2017.09.005
Ozaki, Manami ; Takayama, Tadahiro ; Yamamoto, Takanobu ; Ozawa, Yasumasa ; Nagao, Mayu ; Tanabe, Natsuko ; Nakajima, Akira ; Suzuki, Naoto ; Maeno, Masao ; Yamano, Seiichi ; Sato, Shuichi. / A collagen membrane containing osteogenic protein-1 facilitates bone regeneration in a rat mandibular bone defect. In: Archives of Oral Biology. 2017 ; Vol. 84. pp. 19-28.
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abstract = "Objectives Osteogenic protein-1 (OP-1) has shown osteoinductive activities and is useful for clinical treatments, including bone regeneration. Regenerative procedures using a bioabsorbable collagen membrane (BCM) are well established in periodontal and implant dentistry. We evaluated the subsequent effects of the BCM in combination with OP-1 on bone regeneration in a rat mandibular circular critical-sized bone defect in vivo. Design We used 8 rats that received surgery in both sides of the mandible, and created the total 16 defects which were divided into 4 groups: Group 1; no treatment, as a control, Group 2; BCM alone, Group 3; BCM containing low dose 0.5 μg of OP-1 (L-OP-1), and Group 4; BCM containing high dose 2.0 μg of OP-1 (H-OP-1). Newly formed bone was evaluated by micro computed tomography (micro-CT) and histological analyses at 8 weeks postoperatively. In quantitative and qualitative micro-CT analyses of the volume of new bone formation, bone density, and percentage of new bone area was evaluated. Results BCM with rhOP-1 significantly increased and accelerated bone volume, bone mineral density, and percentage of new bone area compared to control and BCM alone at 8 weeks after surgery; these enhancements in bone regeneration in the OP-1-treated groups were dose-dependent. Conclusions OP-1 delivered with a BCM may have effective osteoinductive potency and be a good combination for bone regeneration. The use of such a combination device for osteogenesis may result in safer and more predictable bone regenerative outcomes in the future.",
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AU - Tanabe, Natsuko

AU - Nakajima, Akira

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AU - Yamano, Seiichi

AU - Sato, Shuichi

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N2 - Objectives Osteogenic protein-1 (OP-1) has shown osteoinductive activities and is useful for clinical treatments, including bone regeneration. Regenerative procedures using a bioabsorbable collagen membrane (BCM) are well established in periodontal and implant dentistry. We evaluated the subsequent effects of the BCM in combination with OP-1 on bone regeneration in a rat mandibular circular critical-sized bone defect in vivo. Design We used 8 rats that received surgery in both sides of the mandible, and created the total 16 defects which were divided into 4 groups: Group 1; no treatment, as a control, Group 2; BCM alone, Group 3; BCM containing low dose 0.5 μg of OP-1 (L-OP-1), and Group 4; BCM containing high dose 2.0 μg of OP-1 (H-OP-1). Newly formed bone was evaluated by micro computed tomography (micro-CT) and histological analyses at 8 weeks postoperatively. In quantitative and qualitative micro-CT analyses of the volume of new bone formation, bone density, and percentage of new bone area was evaluated. Results BCM with rhOP-1 significantly increased and accelerated bone volume, bone mineral density, and percentage of new bone area compared to control and BCM alone at 8 weeks after surgery; these enhancements in bone regeneration in the OP-1-treated groups were dose-dependent. Conclusions OP-1 delivered with a BCM may have effective osteoinductive potency and be a good combination for bone regeneration. The use of such a combination device for osteogenesis may result in safer and more predictable bone regenerative outcomes in the future.

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