A Cellular Resolution Map of Barrel Cortex Activity during Tactile Behavior

Simon Peron, Jeremy Freeman, Vijay Iyer, Caiying Guo, Karel Svoboda

Research output: Contribution to journalArticle

Abstract

Comprehensive measurement of neural activity remains challenging due to the large numbers of neurons in each brain area. We used volumetric two-photon imaging in mice expressing GCaMP6s and nuclear red fluorescent proteins to sample activity in 75% of superficial barrel cortex neurons across the relevant cortical columns, approximately 12,000 neurons per animal, during performance of a single whisker object localization task. Task-related activity peaked during object palpation. An encoding modelrelated activity to behavioral variables. In the column corresponding to the spared whisker, 300 layer (L) 2/3 pyramidal neurons (17%) each encoded touch and whisker movements. Touch representation declined by half in surrounding columns; whisker movement representation was unchanged. Following the emergence of stereotyped task-related movement, sensory representations showed no measurable plasticity. Touch direction was topographically organized, with distinct organization for passive and active touch. Our work reveals sparse and spatially intermingled representations of multiple tactile features. Video Abstract: Peron etal. comprehensively sample activity in superficial barrel cortex of behaving mice using two-photon calcium imaging. They demonstrate sparse and spatially intermingled representations of multiple tactile features and show that these representations are stable during learning.

Original languageEnglish (US)
Pages (from-to)783-799
Number of pages17
JournalNeuron
Volume86
Issue number3
DOIs
StatePublished - May 6 2015

Fingerprint

Touch
Vibrissae
Photons
Neurons
Pyramidal Cells
Palpation
Learning
Calcium
Brain

ASJC Scopus subject areas

  • Neuroscience(all)
  • Medicine(all)

Cite this

A Cellular Resolution Map of Barrel Cortex Activity during Tactile Behavior. / Peron, Simon; Freeman, Jeremy; Iyer, Vijay; Guo, Caiying; Svoboda, Karel.

In: Neuron, Vol. 86, No. 3, 06.05.2015, p. 783-799.

Research output: Contribution to journalArticle

Peron, Simon ; Freeman, Jeremy ; Iyer, Vijay ; Guo, Caiying ; Svoboda, Karel. / A Cellular Resolution Map of Barrel Cortex Activity during Tactile Behavior. In: Neuron. 2015 ; Vol. 86, No. 3. pp. 783-799.
@article{2bc7c20407394454855602ae1483f1d7,
title = "A Cellular Resolution Map of Barrel Cortex Activity during Tactile Behavior",
abstract = "Comprehensive measurement of neural activity remains challenging due to the large numbers of neurons in each brain area. We used volumetric two-photon imaging in mice expressing GCaMP6s and nuclear red fluorescent proteins to sample activity in 75{\%} of superficial barrel cortex neurons across the relevant cortical columns, approximately 12,000 neurons per animal, during performance of a single whisker object localization task. Task-related activity peaked during object palpation. An encoding modelrelated activity to behavioral variables. In the column corresponding to the spared whisker, 300 layer (L) 2/3 pyramidal neurons (17{\%}) each encoded touch and whisker movements. Touch representation declined by half in surrounding columns; whisker movement representation was unchanged. Following the emergence of stereotyped task-related movement, sensory representations showed no measurable plasticity. Touch direction was topographically organized, with distinct organization for passive and active touch. Our work reveals sparse and spatially intermingled representations of multiple tactile features. Video Abstract: Peron etal. comprehensively sample activity in superficial barrel cortex of behaving mice using two-photon calcium imaging. They demonstrate sparse and spatially intermingled representations of multiple tactile features and show that these representations are stable during learning.",
author = "Simon Peron and Jeremy Freeman and Vijay Iyer and Caiying Guo and Karel Svoboda",
year = "2015",
month = "5",
day = "6",
doi = "10.1016/j.neuron.2015.03.027",
language = "English (US)",
volume = "86",
pages = "783--799",
journal = "Neuron",
issn = "0896-6273",
publisher = "Cell Press",
number = "3",

}

TY - JOUR

T1 - A Cellular Resolution Map of Barrel Cortex Activity during Tactile Behavior

AU - Peron, Simon

AU - Freeman, Jeremy

AU - Iyer, Vijay

AU - Guo, Caiying

AU - Svoboda, Karel

PY - 2015/5/6

Y1 - 2015/5/6

N2 - Comprehensive measurement of neural activity remains challenging due to the large numbers of neurons in each brain area. We used volumetric two-photon imaging in mice expressing GCaMP6s and nuclear red fluorescent proteins to sample activity in 75% of superficial barrel cortex neurons across the relevant cortical columns, approximately 12,000 neurons per animal, during performance of a single whisker object localization task. Task-related activity peaked during object palpation. An encoding modelrelated activity to behavioral variables. In the column corresponding to the spared whisker, 300 layer (L) 2/3 pyramidal neurons (17%) each encoded touch and whisker movements. Touch representation declined by half in surrounding columns; whisker movement representation was unchanged. Following the emergence of stereotyped task-related movement, sensory representations showed no measurable plasticity. Touch direction was topographically organized, with distinct organization for passive and active touch. Our work reveals sparse and spatially intermingled representations of multiple tactile features. Video Abstract: Peron etal. comprehensively sample activity in superficial barrel cortex of behaving mice using two-photon calcium imaging. They demonstrate sparse and spatially intermingled representations of multiple tactile features and show that these representations are stable during learning.

AB - Comprehensive measurement of neural activity remains challenging due to the large numbers of neurons in each brain area. We used volumetric two-photon imaging in mice expressing GCaMP6s and nuclear red fluorescent proteins to sample activity in 75% of superficial barrel cortex neurons across the relevant cortical columns, approximately 12,000 neurons per animal, during performance of a single whisker object localization task. Task-related activity peaked during object palpation. An encoding modelrelated activity to behavioral variables. In the column corresponding to the spared whisker, 300 layer (L) 2/3 pyramidal neurons (17%) each encoded touch and whisker movements. Touch representation declined by half in surrounding columns; whisker movement representation was unchanged. Following the emergence of stereotyped task-related movement, sensory representations showed no measurable plasticity. Touch direction was topographically organized, with distinct organization for passive and active touch. Our work reveals sparse and spatially intermingled representations of multiple tactile features. Video Abstract: Peron etal. comprehensively sample activity in superficial barrel cortex of behaving mice using two-photon calcium imaging. They demonstrate sparse and spatially intermingled representations of multiple tactile features and show that these representations are stable during learning.

UR - http://www.scopus.com/inward/record.url?scp=84927164590&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84927164590&partnerID=8YFLogxK

U2 - 10.1016/j.neuron.2015.03.027

DO - 10.1016/j.neuron.2015.03.027

M3 - Article

VL - 86

SP - 783

EP - 799

JO - Neuron

JF - Neuron

SN - 0896-6273

IS - 3

ER -