A C version of Fourier-derived affinity spectrum analysis (FASA) to resolve binding heterogeneity

J. I. Mechanick, Charles Peskin

Research output: Contribution to journalArticle

Abstract

The computer program described in this paper facilitates resolution of binding affinity heterogeneity by transforming binding curve data (bound versus free) into affinity spectra (density versus affinity). The original program, written in FORTRAN, is extended and presented here in the language C. New applications include an ability to transform competition curves into affinity spectra and to evaluate the effects of sampling and experimental error on spectram analysis. We propose that this program be incorporated in the routine evaluation of binding systems.

Original languageEnglish (US)
Pages (from-to)189-194
Number of pages6
JournalComputer Applications in the Biosciences
Volume6
Issue number3
StatePublished - 1990

Fingerprint

Spectrum Analysis
Aptitude
Selection Bias
Spectrum analysis
Affine transformation
Computer program listings
Resolve
Language
Software
Sampling
D.3.2 [Programming Languages]: Language Classifications - Fortran
Curve
Transform
Evaluate
Evaluation

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Molecular Biology
  • Statistics and Probability
  • Computational Mathematics
  • Computer Science Applications
  • Computational Theory and Mathematics
  • Biochemistry

Cite this

A C version of Fourier-derived affinity spectrum analysis (FASA) to resolve binding heterogeneity. / Mechanick, J. I.; Peskin, Charles.

In: Computer Applications in the Biosciences, Vol. 6, No. 3, 1990, p. 189-194.

Research output: Contribution to journalArticle

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