A broad-spectrum microbicide with virucidal activity against sexually transmitted viruses

M. K. Howett, E. B. Neely, N. D. Christensen, B. Wigdahl, F. C. Krebs, Daniel Malamud, S. D. Patrick, M. D. Pickel, P. A. Welsh, C. A. Reed, M. G. Ward, L. R. Budgeon, J. W. Kreider

Research output: Contribution to journalArticle

Abstract

Sodium dodecyl sulfate (SDS), an alkyl sulfate surfactant derived from an organic alcohol, possesses surfactant properties but also denatures and unfolds both monomeric and subunit proteins. In preliminary experiments, we demonstrated that SDS is a potent inactivator of herpes simplex virus type 2 and human immunodeficiency virus type 1 at concentrations comparable to those used for the surfactant nonoxynol-9. We hypothesized thai SDS might be capable of denaturing the capsid proteins of nonenveloped viruses. In this report, we demonstrate inactivation of rabbit, bovine, and human papillomaviruses after brief treatment with dilute solutions of SDS. Effective concentrations were nontoxic to rabbit skin and to split-thickness grafts of human foreskin epithelium. This is the first report of a microbicidal surfactant that will inactivate papillomaviruses. We propose that SDS is now a candidate microbicide for formulation and testing with humans.

Original languageEnglish (US)
Pages (from-to)314-321
Number of pages8
JournalAntimicrobial Agents and Chemotherapy
Volume43
Issue number2
StatePublished - Feb 1999

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Anti-Infective Agents
Sodium Dodecyl Sulfate
Surface-Active Agents
Viruses
Nonoxynol
Rabbits
Foreskin
Human Herpesvirus 2
Protein Subunits
Capsid Proteins
Sulfates
HIV-1
Epithelium
Alcohols
Transplants
Skin

ASJC Scopus subject areas

  • Pharmacology (medical)

Cite this

Howett, M. K., Neely, E. B., Christensen, N. D., Wigdahl, B., Krebs, F. C., Malamud, D., ... Kreider, J. W. (1999). A broad-spectrum microbicide with virucidal activity against sexually transmitted viruses. Antimicrobial Agents and Chemotherapy, 43(2), 314-321.

A broad-spectrum microbicide with virucidal activity against sexually transmitted viruses. / Howett, M. K.; Neely, E. B.; Christensen, N. D.; Wigdahl, B.; Krebs, F. C.; Malamud, Daniel; Patrick, S. D.; Pickel, M. D.; Welsh, P. A.; Reed, C. A.; Ward, M. G.; Budgeon, L. R.; Kreider, J. W.

In: Antimicrobial Agents and Chemotherapy, Vol. 43, No. 2, 02.1999, p. 314-321.

Research output: Contribution to journalArticle

Howett, MK, Neely, EB, Christensen, ND, Wigdahl, B, Krebs, FC, Malamud, D, Patrick, SD, Pickel, MD, Welsh, PA, Reed, CA, Ward, MG, Budgeon, LR & Kreider, JW 1999, 'A broad-spectrum microbicide with virucidal activity against sexually transmitted viruses', Antimicrobial Agents and Chemotherapy, vol. 43, no. 2, pp. 314-321.
Howett, M. K. ; Neely, E. B. ; Christensen, N. D. ; Wigdahl, B. ; Krebs, F. C. ; Malamud, Daniel ; Patrick, S. D. ; Pickel, M. D. ; Welsh, P. A. ; Reed, C. A. ; Ward, M. G. ; Budgeon, L. R. ; Kreider, J. W. / A broad-spectrum microbicide with virucidal activity against sexually transmitted viruses. In: Antimicrobial Agents and Chemotherapy. 1999 ; Vol. 43, No. 2. pp. 314-321.
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