5-HT(1A) receptor localization on the axon hillock of cervical spinal motoneurons in primates

N. M. Kheck, P. J. Gannon, E. C. Azmitia

Research output: Contribution to journalArticle

Abstract

Serotonin (5-HT) has direct and specific effects on the activity of spinal cord motoneurons. The 5-HT(1A) receptor has been shown to mediate motoneuron responses in spinal reflex pathways using the highly selective 5-HT(1A) receptor agonist 8-OH-DPAT. We have developed an antipeptide antibody that recognizes a specific region (the second external loop) of the 5-HT(1A) receptor. This 5-HT(1A) receptor antibody labels populations of neurons and glia in the primate cervical spinal cord. The highest receptor density is present in the superficial lamina of the dorsal horn, around the central canal, and on the axon hillock of large ventral horn motoneurons. The cellular labeling pattern on motoneurons shows a single, densely stained, tapering process emanating from the perikaryon. A more diffuse label is also present throughout the soma. Dendritic labeling was not apparent. These results suggest that post-synaptic 5-HT(1A) receptors may be involved in modulating spinal motoneuron activity at the key site of action potential initiation, the axon hillock.

Original languageEnglish (US)
Pages (from-to)211-220
Number of pages10
JournalJournal of Comparative Neurology
Volume355
Issue number2
StatePublished - 1995

Fingerprint

Receptor, Serotonin, 5-HT1A
Motor Neurons
Primates
Serotonin
8-Hydroxy-2-(di-n-propylamino)tetralin
Antibodies
Carisoprodol
Horns
Neuroglia
Action Potentials
Reflex
Spinal Cord
Axon Initial Segment
Neurons
Population

Keywords

  • autoreceptor
  • immunocytochemistry
  • Macaca
  • receptor antibody
  • serotonin

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

5-HT(1A) receptor localization on the axon hillock of cervical spinal motoneurons in primates. / Kheck, N. M.; Gannon, P. J.; Azmitia, E. C.

In: Journal of Comparative Neurology, Vol. 355, No. 2, 1995, p. 211-220.

Research output: Contribution to journalArticle

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