[3H]5-Hydroxytryptamine binding to brain astroglial cells: Differences between intact and homogenized preparations and mature and immature cultures

P. M. Whitaker-Azmitia, Efrain Azmitia

Research output: Contribution to journalArticle

Abstract

[3H]5-hydroxytryptamine ([3H]serotonin) binds with high affinity (K(D) 2-12 nM) to a finite number of sites on brain astroglial cells. The number of binding sites in the C6 glioma line is decreased significantly (B(max) = 315 versus 30 fmol/mg) by homogenization. In intact primary cultures, derived from newborn rat brain, the number of binding sites is far greater in cultures of immature astrocytes than in cultures treated with dibutyryl cyclic AMP (B(max) 1,520 versus 580 fmol/mg). A role for these receptors in development is suggested.

Original languageEnglish (US)
Pages (from-to)1186-1189
Number of pages4
JournalJournal of Neurochemistry
Volume46
Issue number4
StatePublished - 1986

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Brain
Serotonin
Binding Sites
Bucladesine
Glioma
Astrocytes
Rats

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

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abstract = "[3H]5-hydroxytryptamine ([3H]serotonin) binds with high affinity (K(D) 2-12 nM) to a finite number of sites on brain astroglial cells. The number of binding sites in the C6 glioma line is decreased significantly (B(max) = 315 versus 30 fmol/mg) by homogenization. In intact primary cultures, derived from newborn rat brain, the number of binding sites is far greater in cultures of immature astrocytes than in cultures treated with dibutyryl cyclic AMP (B(max) 1,520 versus 580 fmol/mg). A role for these receptors in development is suggested.",
author = "Whitaker-Azmitia, {P. M.} and Efrain Azmitia",
year = "1986",
language = "English (US)",
volume = "46",
pages = "1186--1189",
journal = "Journal of Neurochemistry",
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AU - Azmitia, Efrain

PY - 1986

Y1 - 1986

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AB - [3H]5-hydroxytryptamine ([3H]serotonin) binds with high affinity (K(D) 2-12 nM) to a finite number of sites on brain astroglial cells. The number of binding sites in the C6 glioma line is decreased significantly (B(max) = 315 versus 30 fmol/mg) by homogenization. In intact primary cultures, derived from newborn rat brain, the number of binding sites is far greater in cultures of immature astrocytes than in cultures treated with dibutyryl cyclic AMP (B(max) 1,520 versus 580 fmol/mg). A role for these receptors in development is suggested.

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