3D conductive nanocomposite scaffold for bone tissue engineering

Aref Shahini, Mostafa Yazdimamaghani, Kenneth J. Walker, Margaret A. Eastman, Hamed Hatami-Marbini, Brenda J. Smith, John Ricci, Sundar V. Madihally, Daryoosh Vashaee, Lobat Tayebi

Research output: Contribution to journalArticle

Abstract

Bone healing can be significantly expedited by applying electrical stimuli in the injured region. Therefore, a three-dimensional (3D) ceramic conductive tissue engineering scaffold for large bone defects that can locally deliver the electrical stimuli is highly desired. In the present study, 3D conductive scaffolds were prepared by employing a biocompatible conductive polymer, ie, poly(3,4-ethylenedioxythiophene) poly(4-styrene sulfonate) (PEDOT:PSS), in the optimized nanocomposite of gelatin and bioactive glass. For in vitro analysis, adult human mesenchymal stem cells were seeded in the scaffolds. Material characterizations using hydrogen-1 nuclear magnetic resonance, in vitro degradation, as well as thermal and mechanical analysis showed that incorporation of PEDOT:PSS increased the physiochemical stability of the composite, resulting in improved mechanical properties and biodegradation resistance. The outcomes indicate that PEDOT:PSS and polypeptide chains have close interaction, most likely by forming salt bridges between arginine side chains and sulfonate groups. The morphology of the scaffolds and cultured human mesenchymal stem cells were observed and analyzed via scanning electron microscope, micro-computed tomography, and confocal fluorescent microscope. Increasing the concentration of the conductive polymer in the scaffold enhanced the cell viability, indicating the improved microstructure of the scaffolds or boosted electrical signaling among cells. These results show that these conductive scaffolds are not only structurally more favorable for bone tissue engineering, but also can be a step forward in combining the tissue engineering techniques with the method of enhancing the bone healing by electrical stimuli.

Original languageEnglish (US)
Pages (from-to)167-181
Number of pages15
JournalInternational Journal of Nanomedicine
Volume9
Issue number1
DOIs
StatePublished - Dec 24 2013

Fingerprint

Nanocomposites
Tissue Engineering
Scaffolds (biology)
Tissue engineering
Scaffolds
Styrene
Bone
Bone and Bones
Mesenchymal Stromal Cells
Polymers
Tissue Scaffolds
Stem cells
Ceramics
Gelatin
Cell signaling
Glass
Arginine
Hydrogen
Cell Survival
Bioactive glass

Keywords

  • Bioactive glass nanoparticles
  • Bone scaffold
  • Conductive polymers
  • Conductive scaffold
  • Gelatin
  • PEDOT: PSS

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Organic Chemistry
  • Drug Discovery

Cite this

Shahini, A., Yazdimamaghani, M., Walker, K. J., Eastman, M. A., Hatami-Marbini, H., Smith, B. J., ... Tayebi, L. (2013). 3D conductive nanocomposite scaffold for bone tissue engineering. International Journal of Nanomedicine, 9(1), 167-181. https://doi.org/10.2147/IJN.S54668

3D conductive nanocomposite scaffold for bone tissue engineering. / Shahini, Aref; Yazdimamaghani, Mostafa; Walker, Kenneth J.; Eastman, Margaret A.; Hatami-Marbini, Hamed; Smith, Brenda J.; Ricci, John; Madihally, Sundar V.; Vashaee, Daryoosh; Tayebi, Lobat.

In: International Journal of Nanomedicine, Vol. 9, No. 1, 24.12.2013, p. 167-181.

Research output: Contribution to journalArticle

Shahini, A, Yazdimamaghani, M, Walker, KJ, Eastman, MA, Hatami-Marbini, H, Smith, BJ, Ricci, J, Madihally, SV, Vashaee, D & Tayebi, L 2013, '3D conductive nanocomposite scaffold for bone tissue engineering', International Journal of Nanomedicine, vol. 9, no. 1, pp. 167-181. https://doi.org/10.2147/IJN.S54668
Shahini A, Yazdimamaghani M, Walker KJ, Eastman MA, Hatami-Marbini H, Smith BJ et al. 3D conductive nanocomposite scaffold for bone tissue engineering. International Journal of Nanomedicine. 2013 Dec 24;9(1):167-181. https://doi.org/10.2147/IJN.S54668
Shahini, Aref ; Yazdimamaghani, Mostafa ; Walker, Kenneth J. ; Eastman, Margaret A. ; Hatami-Marbini, Hamed ; Smith, Brenda J. ; Ricci, John ; Madihally, Sundar V. ; Vashaee, Daryoosh ; Tayebi, Lobat. / 3D conductive nanocomposite scaffold for bone tissue engineering. In: International Journal of Nanomedicine. 2013 ; Vol. 9, No. 1. pp. 167-181.
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