3D arrays for high throughput assay of cell migration and electrotaxis

Sanjun Zhao, Runchi Gao, Peter N. Devreotes, Alex Mogilner, Min Zhao

Research output: Contribution to journalArticle

Abstract

Cell behaviour in 3D environments can be significantly different from those in 2D cultures. With many different 3D matrices being developed and many experimental modalities used to modulate cell behaviour in 3D, it is necessary to develop high throughput techniques to study behaviour in 3D. We report on a 3D array on slide and have adapted this to our electrotaxis chamber, thereby offering a novel approach to quantify cellular responses to electric fields (EFs) in 3D conditions, in different matrices, with different strains of cells, under various field strengths. These developments used Dictyostelium cells to illustrate possible applications and limitations.

Original languageEnglish (US)
Pages (from-to)995-1002
Number of pages8
JournalCell Biology International
Volume37
Issue number9
DOIs
StatePublished - May 13 2013

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Keywords

  • 3D matrices
  • Array
  • Assay
  • Cell locomotion
  • Electrotaxis
  • Morphology

ASJC Scopus subject areas

  • Cell Biology

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