18S ribosomal RNA gene phylogeny for some rhabditidae related to Caenorhabditis

D. H A Fitch, B. Bugaj-Gaweda, S. W. Emmons

Research output: Contribution to journalArticle

Abstract

We have investigated the molecular evolution of the nucleotide sequences of 18S ribosomal RNA genes (18S rDNA) from a set of nematodes in the family Rhabditidae (Nematoda: Secernentea). Our aim was to evaluate the usefulness of this gene for molecular systematics of this family, as well as to establish phylogenetic relationships within a group that has potential for comparative studies of the relationship between development and evolution. We determined the 18S rDNA sequences of nine species of nematodes representing six genera within this family (Caenorhabditis briggsae, C. vulgaris, C. remanei, Rhabditis blumi, Rhabditis sp. br, Rhabditella axei, Pellioditis typica, Teratorhabditis palmarum, and Pelodera strongyloides dermatitica). Using hypothetical models for secondary structure as well as nucleotide similarity, these sequences were aligned with the 18S rDNA sequence published by Ellis et al. for C. elegans and with the partial sequences published by Nadler for eight ascaridoid species. We find that 18S rDNA is likely to be a useful tool to resolve relationships at the intrafamilial level. However, 18S rDNA sequences cannot be used to resolve relationships between taxa as closely related as the Caenorhabditis species. Parsimony, minimum-evolution, and maximum-likelihood methods strongly reject Andrassy's proposed phylogenetic classification based on adult morphological characters but support that of Sudhaus as one alternative of a few possible phylogenies. Distances between genera in this family are about eight times as great as distances between tetrapod classes, suggesting rapid rates of substitution, ancient divergence, or both.

Original languageEnglish (US)
Pages (from-to)346-358
Number of pages13
JournalMolecular Biology and Evolution
Volume12
Issue number2
StatePublished - 1995

Fingerprint

Caenorhabditis
18S Ribosomal RNA
Rhabditidae
Phylogeny
Ribosomal DNA
rRNA Genes
Rhabditis
RNA
phylogeny
Genes
Nematoda
ribosomal RNA
Rhabditoidea
gene
Caenorhabditis briggsae
nematode
Strongyloides
Secernentea
genes
molecular systematics

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Ecology, Evolution, Behavior and Systematics
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Genetics
  • Molecular Biology
  • Genetics(clinical)

Cite this

18S ribosomal RNA gene phylogeny for some rhabditidae related to Caenorhabditis. / Fitch, D. H A; Bugaj-Gaweda, B.; Emmons, S. W.

In: Molecular Biology and Evolution, Vol. 12, No. 2, 1995, p. 346-358.

Research output: Contribution to journalArticle

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