115 kDa protein from Xenopus laevis embryos recognized by antibodies directed against the Xenopus homeoprotein XIHbox 1

M. Flavin, J. P. Saint-Jeannet, A. M. Duprat, F. Strauss

Research output: Contribution to journalArticle

Abstract

Using antibodies against homeoprotein XIHbox 1 from Xenopus laevis, we have detected a new embryonic protein with a much larger molecular weight, 115 kDa. Antibodies fractionated according to their affinity for 3 different domains of the XIHbox 1 protein were used to show that this new protein is related to the C-terminal region of XIHbox 1 protein, downstream from the homeodomain. By immunohistochemistry, the protein was shown to be localized in nuclei of embryonic cells. On SDS-polyacrylamide gels, the 115 kDa protein appears as a set of closely spaced bands whose pattern varies with the stage of development and with the parental origin of the embryos. The protein could be extracted from embryos in a multiprotein complex of approximately 600 kDa. In contrast, the 18 and 27 kDa proteins predicted from the sequence of cloned cDNA to be transcribed and translated from the XIHbox 1 gene could not be detected, suggesting that they are rare or unstable in embryos. These data suggest that the new protein is involved in the development of Xenopus embryos, with a function possibly related to that of the homeoprotein XIHbox 1.

Original languageEnglish (US)
Pages (from-to)309-315
Number of pages7
JournalInternational Journal of Developmental Biology
Volume39
Issue number2
StatePublished - Jan 1 1995

Keywords

  • Xenopus embryogenesis
  • homeobox
  • homeodomain protein
  • immunoblot
  • immunohistochemistry

ASJC Scopus subject areas

  • Embryology
  • Developmental Biology

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