β1 Integrin-mediated collagen gel contraction is stimulated by PDGF

Donald Gullberg, Anders Tingström, Ann Charlotte Thuresson, Lennart Olsson, Louis Terracio, Thomas K. Borg, Kristofer Rubin

Research output: Contribution to journalArticle

Abstract

The attachment of primary rat hepatocytes and fibroblasts to collagen type I is mediated by non-RGD-dependent β1 integrin matrix receptors. In this report we describe a novel 96-well microtiter plate assay for the quantification of fibroblast-mediated contraction of floating collagen type I gels. Fetal calf serum and platelet-derived growth factor (PDGF), but not transforming growth factor-β1, stimulated primary rat heart fibroblasts and normal human diploid fibroblasts (AG 1518) to contract collagen gels to less than 10% of the initial gel volume within a 24-h incubation period. Rabbit polyclonal antibodies directed to the rat hepatocyte integrin β1-chain inhibited the PDGF-stimulated collagen gel contraction. The inhibitory activity on contraction of the anti-β1 integrin IgG could be overcome by adding higher doses of PDGF. The contraction process was not blocked by anti-fibronectin IgG nor by synthetic peptides containing the tripeptide Arg-Gly-Asp (RGD), in concentrations that readily blocked fibroblast attachment to fibronectin-coated planar substrates. Autologous fibronectin or control peptides containing the tripeptide Arg-Gly-Glu were without effect. Immunofluorescence microscopy on fibroblasts grown within collagen gels revealed a punctate distribution of the β1 integrin and a lack of detectable levels of endogenously produced fibronectin. Collectively these data suggest a role for integrin collagen receptors with affinity for collagen fibers, distinct from the previously described RGD-dependent fibronectin receptors, in the fibronectin-independent PDGF-stimulated collagen gel contraction process.

Original languageEnglish (US)
Pages (from-to)264-272
Number of pages9
JournalExperimental Cell Research
Volume186
Issue number2
DOIs
StatePublished - 1990

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Platelet-Derived Growth Factor
Integrins
Fibronectins
Collagen
Fibroblasts
Gels
Collagen Type I
arginyl-glycyl-glutamic acid
Hepatocytes
Fibronectin Receptors
Collagen Receptors
Peptides
Transforming Growth Factors
Diploidy
Fluorescence Microscopy
Immunoglobulin G
Rabbits
Antibodies
Serum

ASJC Scopus subject areas

  • Cell Biology

Cite this

Gullberg, D., Tingström, A., Thuresson, A. C., Olsson, L., Terracio, L., Borg, T. K., & Rubin, K. (1990). β1 Integrin-mediated collagen gel contraction is stimulated by PDGF. Experimental Cell Research, 186(2), 264-272. https://doi.org/10.1016/0014-4827(90)90305-T

β1 Integrin-mediated collagen gel contraction is stimulated by PDGF. / Gullberg, Donald; Tingström, Anders; Thuresson, Ann Charlotte; Olsson, Lennart; Terracio, Louis; Borg, Thomas K.; Rubin, Kristofer.

In: Experimental Cell Research, Vol. 186, No. 2, 1990, p. 264-272.

Research output: Contribution to journalArticle

Gullberg, D, Tingström, A, Thuresson, AC, Olsson, L, Terracio, L, Borg, TK & Rubin, K 1990, 'β1 Integrin-mediated collagen gel contraction is stimulated by PDGF', Experimental Cell Research, vol. 186, no. 2, pp. 264-272. https://doi.org/10.1016/0014-4827(90)90305-T
Gullberg, Donald ; Tingström, Anders ; Thuresson, Ann Charlotte ; Olsson, Lennart ; Terracio, Louis ; Borg, Thomas K. ; Rubin, Kristofer. / β1 Integrin-mediated collagen gel contraction is stimulated by PDGF. In: Experimental Cell Research. 1990 ; Vol. 186, No. 2. pp. 264-272.
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